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Literature DB >> 1100205

Immunofluorescent cell assay of neonatal calf diarrhea virus.

B B Barnett, R S Spendlove, M W Peterson, L Y Hsu, V A LaSalle, L N Egbert.

Abstract

A reliable plaque assay procedure has not yet been described for the neonatal calf diarrhea virus. Therefore, a previously developed immunofluorescent cell counting procedure was adapted to assay this virus. Adsorption of the virus to bovine kidney cells plateaued at 60 minutes. The optimal staining time was between 20 and 24 hours postinfection. Infected cells begun releasing from the coverslips if the cultures were incubated longer than 24 hours. This procedure has proven successful with virus grown in cell culture as well as virus present in fecal samples.

Entities: Species

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Year: 1975 PMID： 1100205 PMCID： PMC1277498

Source DB: PubMed Journal: Can J Comp Med ISSN： 0008-4050

4 in total

1. Immunofluorescent cell assay of infectious pancreatic necrosis virus.

Authors: K C Tu; R S Spendlove; R W Goede
Journal: Appl Microbiol Date: 1974-03

2. Cell culture studies of a neonatal calf diarrhea virus.

Authors: A B Welch; M J Twiehaus
Journal: Can J Comp Med Date: 1973-07

3. Cell culture propagation of neonatal calf diarrhea (scours) virus.

Authors: C A Mebus; M Kono; N R Underdahl; M J Twiehaus
Journal: Can Vet J Date: 1971-03 Impact factor: 1.008

4. Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests.

Authors: A Z Kapikian; H W Kim; R G Wyatt; W J Rodriguez; S Ross; W L Cline; R H Parrott; R M Chanock
Journal: Science Date: 1974-09-20 Impact factor: 47.728

4 in total

11 in total

1. Effect of enzymes on rotavirus infectivity.

Authors: B B Barnett; R S Spendlove; M L Clark
Journal: J Clin Microbiol Date: 1979-07 Impact factor: 5.948

2. Production of high-titer bovine rotavirus with trypsin.

Authors: S M Clark; B B Barnett; R S Spendlove
Journal: J Clin Microbiol Date: 1979-03 Impact factor: 5.948

3. Characteristics of neonatal calf diarrhea virus ribonucleic acid.

Authors: B B Barnett; L N Egbert; R S Spendlove
Journal: Can J Comp Med Date: 1978-01

4. Trypsin enhancement of rotavirus infectivity: mechanism of enhancement.

Authors: S M Clark; J R Roth; M L Clark; B B Barnett; R S Spendlove
Journal: J Virol Date: 1981-09 Impact factor: 5.103

5. Role of two particle types in bovine rotavirus morphogenesis.

Authors: S M Clark; R S Spendlove; B B Barnett
Journal: J Virol Date: 1980-04 Impact factor: 5.103

6. Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.

Authors: M W Peterson; R S Spendlove; R A Smart
Journal: J Clin Microbiol Date: 1976-03 Impact factor: 5.948

7. Inhibition of rotaviruses by selected antiviral substances: mechanisms of viral inhibition and in vivo activity.

Authors: D F Smee; R W Sidwell; S M Clark; B B Barnett; R S Spendlove
Journal: Antimicrob Agents Chemother Date: 1982-01 Impact factor: 5.191

Review 8. Viral gastroenteritis.

Authors: B Barnett
Journal: Med Clin North Am Date: 1983-09 Impact factor: 5.456

9. Polymerase chain reaction for probe synthesis and for direct amplification in detection of bovine coronavirus.

Authors: A Verbeek; P Tijssen
Journal: J Virol Methods Date: 1990-09 Impact factor: 2.014

10. Detection of bovine enteric coronavirus in clinical specimens by hybridization with cDNA probes.

Authors: A Verbeek; S Dea; P Tijssen
Journal: Mol Cell Probes Date: 1990-04 Impact factor: 2.365