Age-related changes and effect of exercise on the molecular composition of immature equine superficial digital flexor tendons.

To test the hypothesis that exercise at very young age may influence the eventual molecular composition (and hence the biomechanical properties) of tendon tissue in the horse, 43 Dutch Warmblood foals were allotted to 3 differently exercised groups (box-rest, box-rest with training and pasture exercise). Twenty-four superficial digital flexor tendons (SDFTs) were collected at age 5 months (8 from each exercise group) and the others were obtained at 11 months after an additional period of light exercise that was equal for all remaining foals and was intended to see if any induced changes would be reversible or not. Significant changes in DNA content (cellularity), hyaluronic acid (HA) and polysulphated glycosaminoglycans (PSGAGs) were found after the 5 month period of different exercise regimens. There was a tendency towards an exercise-related effect on hydroxylysine content and number of hydroxylysylpyridinoline (HP) crosslinks. Levels of Cartilage Oligomeric Matrix Protein (COMP), measured by homologous inhibition ELISA, showed significant differences at 5 months and were highest in foals kept at pasture and lowest in foals maintained in a box but given enforced exercise. At 11 months, the biochemical parameters of the tendons from the foals of the former box-rest and pasture groups became similar, indicating the capacity of the immature tendon to recover from a retarded development. However, the ratio of PSGAGs per unit of DNA of the former training group was significantly lower than those from the other groups, suggesting that the training regimen in this study had a lasting negative effect on the tenocytes resulting in a decrease of the production of PSGAGs. Therefore, inappropriate or excessive exercise may damage developing tendon, with limited recovery after normalising the exercise level. These possibly deleterious effects of a training regimen on tendon development may be important for the management of young would-be equine athletes.