Improvements in DNA sequencing by capillary electrophoresis at elevated temperature using poly(ethylene oxide) as a sieving matrix.

DNA sequencing in poly(ethylene oxide) (PEO) matrix by capillary electrophoresis was demonstrated at high temperature. The optimal separation temperature is around 40 degrees C. The effects of polymer concentration and types of buffers on the separation performance were investigated. A new buffer system consisting of Tris-Taps-His-EDTA works well with PEO. High-speed separation and good resolution can be fulfilled by using a single-MW PEO polymer. It offers similar separation performance as before for the small DNA fragments, but better performance for large DNA fragments.