Expression of mRNA for axon guidance molecules, such as semaphorin-III, netrins and neurotrophins, in human osteoblasts and osteoclasts.

In the present study, we demonstrated the constitutive expression of diffusible axon guidance molecules such as neurotrophins, semaphorin-III, netrin-1, and netrin-2-like protein, which are known to function as a chemoattractant and/or chemorepellent for growing nerve fibers, in human osteoblastic and osteoclastic cells. The findings, obtained by RT-PCR, ELISA, and Western blot analysis suggest the extension of axons of peripheral sensory and sympathetic neurons to osteoblastic and osteoclastic cells and the possible neural regulation of bone metabolism in these osteogenic cells.