BACKGROUND: Astroviruses (HAstVs) and 'Norwalk-like viruses' (NLV) are frequent causes of gastroenteritis worldwide, though no data on the strains in circulation or their prevalence is available for France. OBJECTIVES: We applied molecular methods to detect HAstVs and NLVs by reverse transcription-polymerase chain reaction (RT-PCR) in fecal samples collected during a 2-year period from children and adults hospitalized with gastroenteritis. STUDY DESIGN: All samples negative for rotavirus and adenovirus by latex agglutination which contained small (25-40 nm) viral particles observed by electron microscopy (EM) were examined by RT-PCR. RT-PCR products were sequenced to characterize the HAstV and NLV strains present. RESULTS: A total of 75 samples were analyzed by RT-PCR, of which 15 were positive for HAstV and 24 for NLV. Several distinct strains of serotype 1 HAstV, the predominant serotype, circulated during the period. Nineteen of the 24 NLVs were of the G2 genogroup including Mexico-like (n=10), Bristol-like (n=8), and Hawaii-like viruses (n=1); two were genogroup 1. Overall, seven (47%) of the 15 HAstV infections and nine (37.5%) of the 24 NLV infections appeared to be nosocomially acquired based on the date of admission in hospital and the date of illness. CONCLUSION: This study provides additional evidence of the importance of nosocomial infections caused by NLV and HAstV.
BACKGROUND: Astroviruses (HAstVs) and 'Norwalk-like viruses' (NLV) are frequent causes of gastroenteritis worldwide, though no data on the strains in circulation or their prevalence is available for France. OBJECTIVES: We applied molecular methods to detect HAstVs and NLVs by reverse transcription-polymerase chain reaction (RT-PCR) in fecal samples collected during a 2-year period from children and adults hospitalized with gastroenteritis. STUDY DESIGN: All samples negative for rotavirus and adenovirus by latex agglutination which contained small (25-40 nm) viral particles observed by electron microscopy (EM) were examined by RT-PCR. RT-PCR products were sequenced to characterize the HAstV and NLV strains present. RESULTS: A total of 75 samples were analyzed by RT-PCR, of which 15 were positive for HAstV and 24 for NLV. Several distinct strains of serotype 1 HAstV, the predominant serotype, circulated during the period. Nineteen of the 24 NLVs were of the G2 genogroup including Mexico-like (n=10), Bristol-like (n=8), and Hawaii-like viruses (n=1); two were genogroup 1. Overall, seven (47%) of the 15 HAstV infections and nine (37.5%) of the 24 NLV infections appeared to be nosocomially acquired based on the date of admission in hospital and the date of illness. CONCLUSION: This study provides additional evidence of the importance of nosocomial infections caused by NLV and HAstV.
Authors: R Tolentino-Ruiz; D Montoya-Varela; M García-Espitia; M Salas-Benito; A Gutiérrez-Escolano; C Gómez-García; P Figueroa-Arredondo; J Salas-Benito; M De Nova-Ocampo Journal: Curr Microbiol Date: 2012-06-19 Impact factor: 2.188
Authors: G La Rosa; S Fontana; A Di Grazia; M Iaconelli; M Pourshaban; M Muscillo Journal: Appl Environ Microbiol Date: 2007-05-04 Impact factor: 4.792
Authors: Sandhya U Parshionikar; Sandra Willian-True; G Shay Fout; David E Robbins; Scott A Seys; Joslyn D Cassady; Richard Harris Journal: Appl Environ Microbiol Date: 2003-09 Impact factor: 4.792
Authors: I Fodha; A Chouikha; I Peenze; M De Beer; J Dewar; A Geyer; F Messaadi; A Trabelsi; N Boujaafar; M B Taylor; D Steele Journal: J Med Virol Date: 2006-09 Impact factor: 2.327
Authors: Marion Koopmans; Carl Henrik von Bonsdorff; Jan Vinjé; Dario de Medici; Steve Monroe Journal: FEMS Microbiol Rev Date: 2002-06 Impact factor: 16.408