| Literature DB >> 10995205 |
B Kühnast1, F Dollé, S Terrazzino, B Rousseau, C Loc'h, F Vaufrey, F Hinnen, I Doignon, F Pillon, C David, C Crouzel, B Tavitian.
Abstract
Evaluation of oligonucleotides for biomedical applications requires different in vivo and in vitro approaches (pharmacokinetics, biodistribution, macro- and microimaging, metabolism,.), that are performed with different radioisotopes according to the temporal and spatial resolution needed. A method to introduce radioactive isotopes of halogens (fluorine, bromine, and iodine) in a small and stable molecule has been developed. Radiosynthons can then be conjugated with any given oligonucleotide in one step to create the appropriate radiotracer. This general radiolabeling procedure for oligonucleotides is efficient to synthesize (18)F-, (76)Br-, and (125)I-oligonucleotides for biological needs. Applications of the method to biodistribution, metabolism, in vivo and ex vivo imaging of (125)I- and (18)F-labeled oligonucleotides are reported.Entities:
Mesh：
Substances：
Year: 2000 PMID： 10995205 DOI： 10.1021/bc990183i
Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774