Literature DB >> 10990065

Immunocytochemical characterization of in vitro PKH26-labelled and intracerebrally transplanted neonatal cells.

S J Haas1, P Bauer, A Rolfs, A Wree.   

Abstract

The lipophilic dye PKH26 that binds irreversibly to cell membranes has been used to label various cell types in vitro prior to transplantation in order to recognize grafted cells posttransplantationally in the host tissue by fluorescence microscopy. The purpose of the present study was to optimize immunocytochemical staining procedures for PKH26-containing specimens in cell culture and after transplantation into rat brain. We demonstrated that freeze-thawing allowed for proper immunostaining of intracellular epitopes whereas PKH26-labelling was preserved. PKH26-labelled donor cells were detectable at least up to 4 months after transplantation in the host brain.

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Year:  2000        PMID： 10990065     DOI： 10.1078/S0065-1281(04)70035-5

Source DB:  PubMed          Journal:  Acta Histochem        ISSN： 0065-1281            Impact factor:   2.479


  9 in total

1.  Orthotopic transplantation of immortalized mesencephalic progenitors (CSM14.1 cells) into the substantia nigra of hemiparkinsonian rats induces neuronal differentiation and motoric improvement.

Authors:  Stefan Jean-Pierre Haas; Stanislav Petrov; Golo Kronenberg; Oliver Schmitt; Andreas Wree
Journal:  J Anat       Date:  2007-11-23       Impact factor: 2.610

2.  Histological study on effect of mesenchymal stem cell therapy on experimental renal injury induced by ischemia/reperfusion in male albino rat.

Authors:  Eman Mostafa Sadek; Noha Mohamed Afifi; Lamiaa Ibrahim Abd Elfattah; Manal Ali Abd-El Mohsen
Journal:  Int J Stem Cells       Date:  2013-05       Impact factor: 2.500

3.  Quinolinic acid lesions of the caudate putamen in the rat lead to a local increase of ciliary neurotrophic factor.

Authors:  Stefan Jean-Pierre Haas; Aline Ahrens; Stanislav Petrov; Oliver Schmitt; Andreas Wree
Journal:  J Anat       Date:  2004-04       Impact factor: 2.610

4.  A Comparison of Exogenous Labels for the Histological Identification of Transplanted Neural Stem Cells.

Authors:  Francesca J Nicholls; Jessie R Liu; Michel Modo
Journal:  Cell Transplant       Date:  2016-11-03       Impact factor: 4.064

5.  Simulated microgravity facilitates cell migration and neuroprotection after bone marrow stromal cell transplantation in spinal cord injury.

Authors:  Takafumi Mitsuhara; Masaaki Takeda; Satoshi Yamaguchi; Tomotaka Manabe; Masaya Matsumoto; Yumi Kawahara; Louis Yuge; Kaoru Kurisu
Journal:  Stem Cell Res Ther       Date:  2013-04-01       Impact factor: 6.832

6.  Differentiation of UC-MSCs into hepatocyte-like cells in partially hepatectomized model rats.

Authors:  Zheng Chen; Qiaoting Kuang; Xue-Jun Lao; Jie Yang; Weidong Huang; Dong Zhou
Journal:  Exp Ther Med       Date:  2016-07-26       Impact factor: 2.447

7.  In vivo imaging system for explants analysis-A new approach for assessment of cell transplantation effects in large animal models.

Authors:  Weronika Zarychta-Wiśniewska; Anna Burdzinska; Radosław Zagozdzon; Bartosz Dybowski; Marta Butrym; Zdzisław Gajewski; Leszek Paczek
Journal:  PLoS One       Date:  2017-09-20       Impact factor: 3.240

8.  Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice.

Authors:  Li Yuan; Hou-Qi Liu; Min-Juan Wu
Journal:  Exp Ther Med       Date:  2016-05-24       Impact factor: 2.447

9.  Therapeutic role of adipose tissue-derived stem cells versus microvesicles in a rat model of cerebellar injury.

Authors:  Nehad F Mazen; Eman A Abdel-Fattah; Shimaa R Desoky; Amal S El-Shal
Journal:  J Cell Mol Med       Date:  2021-12-07       Impact factor: 5.310
  9 in total

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