Literature DB >> 1098730

Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.

J Veldkamp, A M Visser.   

Abstract

The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis.

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Year:  1975        PMID： 1098730      PMCID： PMC1046555          DOI： 10.1136/sti.51.4.227

Source DB:  PubMed          Journal:  Br J Vener Dis        ISSN： 0007-134X


  6 in total

1.  Immunoassay using antigen-enzyme conjugates.

Authors:  B K. Van Weemen; A H.W.M. Schuurs
Journal:  FEBS Lett       Date:  1971-06-24       Impact factor: 4.124

2.  Experiences with the treponemal immobilization test in the Netherlands.

Authors:  J H BEKKER; P C ONVLEE
Journal:  Antonie Van Leeuwenhoek       Date:  1955       Impact factor: 2.271

3.  Isolation and purification of the protein antigen of the Reiter treponeme; a study of its serologic reactions.

Authors:  G D'ALESSANDRO; L DARDANONI
Journal:  Am J Syph Gonorrhea Vener Dis       Date:  1953-03

4.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors:  E Engvall; P Perlmann
Journal:  Immunochemistry       Date:  1971-09

5.  The antigenic structure of Treponema pallidum, Nichols strain. I. The demonstration, nature and location of specific and shared antigens.

Authors:  J N Miller; J H de Bruijn; J H Bekker; P C Onvlee
Journal:  J Immunol       Date:  1966-03       Impact factor: 5.422

6.  Performance and use of the Reiter protein complement-fixation (RPCF) test.

Authors:  J H Bekker; J H de Bruijn; J N Miller
Journal:  Br J Vener Dis       Date:  1966-03
  6 in total
  36 in total

1.  Enzyme immunoassays in diagnostic medicine. Theory and practice.

Authors:  A Voller; D E Bidwell; A Bartlett
Journal:  Bull World Health Organ       Date:  1976       Impact factor: 9.408

Review 2.  Laboratory techniques in the diagnosis of syphilis: a review.

Authors:  J J van der Sluis
Journal:  Genitourin Med       Date:  1992-12

3.  It is time to use treponema-specific antibody screening tests for diagnosis of syphilis.

Authors:  Michael J Loeffelholz; Matthew J Binnicker
Journal:  J Clin Microbiol       Date:  2011-11-16       Impact factor: 5.948

4.  Enzyme immunoassay for anti-treponemal IgG: screening or confirmatory test?

Authors:  H Young; A Moyes; A McMillan; J Patterson
Journal:  J Clin Pathol       Date:  1992-01       Impact factor: 3.411

5.  Rheumatoid factor in syphilis.

Authors:  E H Cerny; C E Farshy; E F Hunter; S A Larsen
Journal:  J Clin Microbiol       Date:  1985-07       Impact factor: 5.948

6.  Solid-phase fluoroimmunoassay for treponemal antibody.

Authors:  R W Stevens; R F Schell
Journal:  J Clin Microbiol       Date:  1982-02       Impact factor: 5.948

7.  Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.

Authors:  V Pope; E F Hunter; J C Feeley
Journal:  J Clin Microbiol       Date:  1982-04       Impact factor: 5.948

Review 8.  Laboratory diagnosis and interpretation of tests for syphilis.

Authors:  S A Larsen; B M Steiner; A H Rudolph
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

9.  Detection of immunoglobulin M in cerebrospinal fluid from syphilis patients by enzyme-linked immunosorbent assay.

Authors:  J B Lee; C E Farshy; E F Hunter; E A Hambie; G H Wobig; S A Larsen
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

10.  Screening for treponemal infection by a new enzyme immunoassay.

Authors:  H Young; A Moyes; A McMillan; D H Robertson
Journal:  Genitourin Med       Date:  1989-04
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