Oscillations of exopolymeric composition and sludge volume index in nitrifying flocs.

Protein, carbohydrate, and lipid composition of the exopolymer fraction of a nitrifying sludge in steady-state culture was analyzed after dissociation with 50 mM EDTA and dialysis of the nonfilamentous flocs. Steady-state culture was established when the nitrification rate was constant. The nitrification efficiency at that regime was 93%, also constant. In steady state the concentration of exopolymer protein in the nitrifying sludge floc oscillated from 5 (lowest) to 45 (highest) mg/L with a consistent oscillating pattern having a duration period of 10 d each. Carbohydrate and lipid content in the flocs showed no significant variations (30 and 36 mg/L, respectively). Only 20% of the extracellular polysaccharides had molecular weights higher than 10 kDa, suggesting that the floc aggregation depended on smaller fractions of low-molecular-weight carbohydrates. The oscillations in the concentration of exopolymeric protein coincided with parallel variations in the sludge volumetric index (SVI) value (12.2+/-2.1 mL/g). Analysis of the polymeric substances of the floc and suspended solids corroborated by statistical analysis indicated that the variations in the SVI of the nitrifying nonfilamentous flocs were mainly related to the changes in the exopolymeric protein content.