Literature DB >> 10981711

Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target.

K Ui-Tei1, S Zenno, Y Miyata, K Saigo.   

Abstract

A sensitive cellular assay system for RNA interference was developed using the firefly luciferase gene as target. RNA interference was noted not only in Drosophila cultured cells but Chinese hamster cells (CHO-K1) as well, although double-stranded RNA required for the latter was 2500 times more than for the former. Cognate double-stranded RNA as short as 38 bp was found to be still capable of inducing RNA interference in Drosophila cultured cells.

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Year:  2000        PMID： 10981711     DOI： 10.1016/s0014-5793(00)01883-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  23 in total

1.  Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate.

Authors:  S M Elbashir; J Martinez; A Patkaniowska; W Lendeckel; T Tuschl
Journal:  EMBO J       Date:  2001-12-03       Impact factor: 11.598

2.  Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines.

Authors:  E Billy; V Brondani; H Zhang; U Müller; W Filipowicz
Journal:  Proc Natl Acad Sci U S A       Date:  2001-11-27       Impact factor: 11.205

3.  Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells.

Authors:  S Yang; S Tutton; E Pierce; K Yoon
Journal:  Mol Cell Biol       Date:  2001-11       Impact factor: 4.272

4.  Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.

Authors:  Kumiko Ui-Tei; Yuki Naito; Fumitaka Takahashi; Takeshi Haraguchi; Hiroko Ohki-Hamazaki; Aya Juni; Ryu Ueda; Kaoru Saigo
Journal:  Nucleic Acids Res       Date:  2004-02-09       Impact factor: 16.971

Review 5.  Structural diversity repertoire of gene silencing small interfering RNAs.

Authors:  Chan Il Chang; Helena Andrade Kim; Pooja Dua; Soyoun Kim; Chiang J Li; Dong-ki Lee
Journal:  Nucleic Acid Ther       Date:  2011-06       Impact factor: 5.486

Review 6.  Therapeutic gene silencing in neurological disorders, using interfering RNA.

Authors:  G Scott Ralph; Nicholas D Mazarakis; Mimoun Azzouz
Journal:  J Mol Med (Berl)       Date:  2005-03-10       Impact factor: 4.599

7.  RNA interference targeting VP1 inhibits foot-and-mouth disease virus replication in BHK-21 cells and suckling mice.

Authors:  Weizao Chen; Weiyao Yan; Qingyun Du; Liang Fei; Mingqiu Liu; Zheng Ni; Zutian Sheng; Zhaoxin Zheng
Journal:  J Virol       Date:  2004-07       Impact factor: 5.103

8.  Adenovirus E1B55K region is required to enhance cyclin E expression for efficient viral DNA replication.

Authors:  Xinyu Zheng; Xiao-Mei Rao; Jorge G Gomez-Gutierrez; Hongying Hao; Kelly M McMasters; H Sam Zhou
Journal:  J Virol       Date:  2008-01-30       Impact factor: 5.103

9.  RNAi induced in mammalian and Drosophila cells via transfection of dimers and trimers of small interfering RNA.

Authors:  Kumiko Ui-Tei; Shuhei Zenno; Aya Juni; Kaoru Saigo
Journal:  J RNAi Gene Silencing       Date:  2005-10-14

10.  Six RNA viruses and forty-one hosts: viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems.

Authors:  Poornima Parameswaran; Ella Sklan; Courtney Wilkins; Trever Burgon; Melanie A Samuel; Rui Lu; K Mark Ansel; Vigo Heissmeyer; Shirit Einav; William Jackson; Tammy Doukas; Suman Paranjape; Charlotta Polacek; Flavia Barreto dos Santos; Roxana Jalili; Farbod Babrzadeh; Baback Gharizadeh; Dirk Grimm; Mark Kay; Satoshi Koike; Peter Sarnow; Mostafa Ronaghi; Shou-Wei Ding; Eva Harris; Marie Chow; Michael S Diamond; Karla Kirkegaard; Jeffrey S Glenn; Andrew Z Fire
Journal:  PLoS Pathog       Date:  2010-02-12       Impact factor: 6.823
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