| Literature DB >> 10978735 |
M E Lanio1, V Morera, C Alvarez, M Tejuca, T Gómez, F Pazos, V Besada, D Martínez, V Huerta, G Padrón, M de los Angeles Chávez.
Abstract
Two hemolysins, Sticholysin I (St I) and Sticholysin II (St II) were purified from the sea anemone Stichodactyla helianthus combining gel filtration and ion exchange chromatography. The amino acid composition of both cytolysins was determined revealing a high proportion of glycine, lysine, tyrosine and non-polar amino acids (alanine, leucine and valine). Cysteine was not found in either polypeptide. Molecular masses of St I and St II were 19401 and 19290 Da, respectively. N-terminal sequence analysis of St I and St II showed a high homology between them suggesting they are isoforms of the same cytolysin. Compared with other sea anemone cytolysins, St I and St II contain a 22 amino acid insertion fragment also present in Eq T II/Tn C and probably in CaT I and Hm T and absent in C III, the major hemolysin previously reported in this anemone.Entities:
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Year: 2001 PMID： 10978735 DOI： 10.1016/s0041-0101(00)00106-9
Source DB: PubMed Journal: Toxicon ISSN： 0041-0101 Impact factor: 3.033