Literature DB >> 10974583

Utility of restriction fragment analysis for typing herpes simplex virus amplicons following PCR of targets in the DNA polymerase gene.

R P Podzorski1, J Baker, J R Merline, R Qureshi, J E Holsinger.   

Abstract

We compared the utility of restriction endonuclease cleavage to type herpes simplex virus DNA polymerase gene amplicons from two well established PCR primer sets. Amplicons typed using Ava II had a 96% correlation to type determined by monoclonal antibody staining, while amplicons typed using Drd I had a 72% correlation to type determined by monoclonal antibody staining.

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Year:  2000        PMID： 10974583     DOI： 10.1016/s0732-8893(00)00153-x

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  1 in total

1.  DNA polymerase gene locus of Cercopithecine herpesvirus 1 is a suitable target for specific and rapid identification of viral infection by PCR technology.

Authors:  Manuel Barreto Miranda; Michaela Handermann; Gholamreza Darai
Journal:  Virus Genes       Date:  2005-05       Impact factor: 2.332
  1 in total

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