Literature DB >> 10972716

Characterization of shiga toxin type 2 variant B-subunit in Escherichia coli strains from asymptomatic human carriers by PCR-RFLP.

R Stephan1, L E Hoelzle.   

Abstract

Subtyping of shiga toxin type 2 variant B-subunit in 35 non-O157 and two O157 strains isolated from 37 asymptomatic human carriers yielded two strains with stx2, 10 strains with stx2c and 24 strains with stx2d genes. One isolate harboured stx2 and stx2c. The high Stx2d prevalence in asymptomatic carriers was conspicuous and may indicate a reduced pathogenicity of these toxin variants. Therefore, in order to appraise a positive STEC laboratory result, the strain must be isolated in every case. Shiga toxin types and further virulence-associated factors have to be investigated.

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Year:  2000        PMID： 10972716     DOI： 10.1046/j.1365-2672.2000.00778.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  13 in total

1.  Presence of activatable Shiga toxin genotype (stx(2d)) in Shiga toxigenic Escherichia coli from livestock sources.

Authors:  Kari S Gobius; Glen M Higgs; Patricia M Desmarchelier
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

2.  Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: type, number of variants, and relationship to cytotoxicity.

Authors:  Alejandra Krüger; Paula M A Lucchesi; Alberto E Parma
Journal:  Appl Environ Microbiol       Date:  2010-10-29       Impact factor: 4.792

3.  Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli.

Authors:  H Schmidt; W L Zhang; U Hemmrich; S Jelacic; W Brunder; P I Tarr; U Dobrindt; J Hacker; H Karch
Journal:  Infect Immun       Date:  2001-11       Impact factor: 3.441

4.  Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factors.

Authors:  Y Bertin; K Boukhors; N Pradel; V Livrelli; C Martin
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

5.  Design of a seven-genome Escherichia coli microarray for comparative genomic profiling.

Authors:  Hanni Willenbrock; Anne Petersen; Camilla Sekse; Kristoffer Kiil; Yngvild Wasteson; David W Ussery
Journal:  J Bacteriol       Date:  2006-09-08       Impact factor: 3.490

Review 6.  Shiga Toxin (Stx) Classification, Structure, and Function.

Authors:  Angela R Melton-Celsa
Journal:  Microbiol Spectr       Date:  2014-08

7.  Analysis of the clonal relationship of shiga toxin-producing Escherichia coli serogroup O165:H25 isolated from cattle.

Authors:  Lutz Geue; Thomas Selhorst; Christina Schnick; Birgit Mintel; Franz J Conraths
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

8.  Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype, and components of the type 2 Shiga toxin family by multiplex PCR.

Authors:  Gehua Wang; Clifford G Clark; Frank G Rodgers
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948

9.  Genetic diversity of heat-labile toxin expressed by enterotoxigenic Escherichia coli strains isolated from humans.

Authors:  M A Lasaro; J F Rodrigues; C Mathias-Santos; B E C Guth; A Balan; M E Sbrogio-Almeida; L C S Ferreira
Journal:  J Bacteriol       Date:  2008-01-25       Impact factor: 3.490

10.  Loop-mediated isothermal amplification assay for detection of generic and verocytotoxin-producing Escherichia coli among indigenous individuals in Malaysia.

Authors:  Cindy Shuan Ju Teh; Kek Heng Chua; Yvonne Ai Lian Lim; Soo Ching Lee; Kwai Lin Thong
Journal:  ScientificWorldJournal       Date:  2014-05-19
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