L J Zhou1, I Ono. 1. Department of Dermatology and Division of Plastic and Reconstructive Surgery, Department of Dermatology, Fukushima Medical University School of Medicine, Hikarigaoka-1, Fukushima 960-1295, Japan.
Abstract
BACKGROUND: Clinical studies have shown that ointment containing dibutyryl cyclic adenosine monophosphate (DBcAMP) promotes wound healing. OBJECTIVES: We aimed to elucidate the mechanisms of the beneficial effect of DBcAMP in wound healing. METHODS: An investigation was made of the effects of DBcAMP on in vitro cytokine release from cultured keratinocytes and fibroblasts derived from normal human skin. RESULTS: DBcAMP stimulated keratinocyte proliferation through increased interleukin (IL)-6 production by fibroblasts, and transiently enhanced production of transforming growth factor (TGF)-beta1 by fibroblasts at an early stage of incubation. DBcAMP also stimulated fibroblast proliferation, resulting in further increases in IL-6 and TGF-beta1. CONCLUSIONS: We conclude that this series of stimulative actions on cytokine secretion, together with the facilitation of cell proliferation, contribute to the effects of DBcAMP on the healing of skin ulcers.
BACKGROUND: Clinical studies have shown that ointment containing dibutyryl cyclic adenosine monophosphate (DBcAMP) promotes wound healing. OBJECTIVES: We aimed to elucidate the mechanisms of the beneficial effect of DBcAMP in wound healing. METHODS: An investigation was made of the effects of DBcAMP on in vitro cytokine release from cultured keratinocytes and fibroblasts derived from normal human skin. RESULTS:DBcAMP stimulated keratinocyte proliferation through increased interleukin (IL)-6 production by fibroblasts, and transiently enhanced production of transforming growth factor (TGF)-beta1 by fibroblasts at an early stage of incubation. DBcAMP also stimulated fibroblast proliferation, resulting in further increases in IL-6 and TGF-beta1. CONCLUSIONS: We conclude that this series of stimulative actions on cytokine secretion, together with the facilitation of cell proliferation, contribute to the effects of DBcAMP on the healing of skin ulcers.
Authors: M Carmen Montesinos; Avani Desai; Jiang-Fan Chen; Herman Yee; Michael A Schwarzschild; J Stephen Fink; Bruce N Cronstein Journal: Am J Pathol Date: 2002-06 Impact factor: 4.307