Literature DB >> 10970295

Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides.

M D Burkart1, M Izumi, E Chapman, C H Lin, C H Wong.   

Abstract

This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyltransferases.

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Year:  2000        PMID： 10970295     DOI： 10.1021/jo000266o

Source DB:  PubMed          Journal:  J Org Chem        ISSN： 0022-3263            Impact factor:   4.354


  27 in total

1.  Determination of enzyme/substrate specificity constants using a multiple substrate ESI-MS assay.

Authors:  Na Pi; Julie A Leary
Journal:  J Am Soc Mass Spectrom       Date:  2004-02       Impact factor: 3.109

2.  Mechanistic studies of beta-arylsulfotransferase IV.

Authors:  Eli Chapman; Marian C Bryan; Chi-Huey Wong
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-23       Impact factor: 11.205

3.  Observation of a hybrid random ping-pong mechanism of catalysis for NodST: a mass spectrometry approach.

Authors:  Na Pi; Yonghao Yu; Joseph D Mougous; Julie A Leary
Journal:  Protein Sci       Date:  2004-04       Impact factor: 6.725

4.  Characterization of noncovalent protein-ligand complexes and associated enzyme intermediates of GlcNAc-6-O-sulfotransferase by electrospray ionization FT-ICR mass spectrometry.

Authors:  Yonghao Yu; Colleen E Kirkup; Na Pi; Julie A Leary
Journal:  J Am Soc Mass Spectrom       Date:  2004-10       Impact factor: 3.109

5.  Expression of heparan sulfate sulfotransferases in Kluyveromyces lactis and preparation of 3'-phosphoadenosine-5'-phosphosulfate.

Authors:  Xianxuan Zhou; Kasemsiri Chandarajoti; Truong Quang Pham; Renpeng Liu; Jian Liu
Journal:  Glycobiology       Date:  2011-01-11       Impact factor: 4.313

6.  Combinatorial one-pot chemoenzymatic synthesis of heparin.

Authors:  Ujjwal Bhaskar; Guoyun Li; Li Fu; Akihiro Onishi; Mathew Suflita; Jonathan S Dordick; Robert J Linhardt
Journal:  Carbohydr Polym       Date:  2014-11-07       Impact factor: 9.381

7.  High cell density cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase for the production of bioengineered heparin.

Authors:  Jianhua Zhang; Matt Suflita; Guoyun Li; Weihong Zhong; Lingyun Li; Jonathan S Dordick; Robert J Linhardt; Fuming Zhang
Journal:  Appl Biochem Biotechnol       Date:  2015-01-14       Impact factor: 2.926

8.  Preparation and Analysis of N-Terminal Chemokine Receptor Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes.

Authors:  Christoph Seibert; Anthony Sanfiz; Thomas P Sakmar; Christopher T Veldkamp
Journal:  Methods Enzymol       Date:  2015-11-14       Impact factor: 1.600

9.  Chemoenzymatic synthesis of homogeneous ultralow molecular weight heparins.

Authors:  Yongmei Xu; Sayaka Masuko; Majde Takieddin; Haoming Xu; Renpeng Liu; Juliana Jing; Shaker A Mousa; Robert J Linhardt; Jian Liu
Journal:  Science       Date:  2011-10-28       Impact factor: 47.728

Review 10.  Heparin and related polysaccharides: synthesis using recombinant enzymes and metabolic engineering.

Authors:  Matthew Suflita; Li Fu; Wenqin He; Mattheos Koffas; Robert J Linhardt
Journal:  Appl Microbiol Biotechnol       Date:  2015-07-29       Impact factor: 4.813
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