Literature DB >> 1096958

Reversibility of the affinity labelled-biotin transport system in yeast cells.

T Viswanatha, E Bayer, M Wilchek.   

Abstract

Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotynyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling of the transport system.

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Year:  1975        PMID: 1096958     DOI: 10.1016/0005-2736(75)90349-1

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  1 in total

1.  Specific localization and quantification of biotin transport components in yeast by use of a biotin-conjugated, impermeant, electron-dense label.

Authors:  E A Bayer; E Skutelsky; T Viswanatha; M Wilchek
Journal:  Mol Cell Biochem       Date:  1978-02-24       Impact factor: 3.396

  1 in total

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