Literature DB >> 10968630

A genetically modified solvent-tolerant bacterium for optimized production of a toxic fine chemical.

J Wery1, D I Mendes da Silva, J A de Bont.   

Abstract

The aim of the study was to investigate whether toxic fine chemical production can be improved using the solvent-tolerant Pseudomonas putida S12 in a two-liquid-phase system consisting of aqueous media and a water-immiscible octanol phase with production of 3-methylcatechol from toluene as the model conversion. For this purpose the genes involved in this conversion, todC1C2BAD from P. putida F1, were introduced into P. putida S12 with high stable expression. Production of 3-methylcatechol was monitored in batch incubations with different media using a single medium and a two-liquid medium-octanol system. The maximum concentration of 3-methylcatechol increased two-fold using the two-liquid medium-octanol system, irrespective of the selected medium.

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Year:  2000        PMID： 10968630     DOI： 10.1007/s002530000381

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  7 in total

1.  Energetics and surface properties of Pseudomonas putida DOT-T1E in a two-phase fermentation system with 1-decanol as second phase.

Authors:  Grit Neumann; Sjef Cornelissen; Frank van Breukelen; Steffi Hunger; Holger Lippold; Norbert Loffhagen; Lukas Y Wick; Hermann J Heipieper
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

2.  Prediction of the adaptability of Pseudomonas putida DOT-T1E to a second phase of a solvent for economically sound two-phase biotransformations.

Authors:  Grit Neumann; Nadja Kabelitz; Andreas Zehnsdorf; Anja Miltner; Holger Lippold; Daniel Meyer; Andreas Schmid; Hermann J Heipieper
Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

3.  Engineering of solvent-tolerant Pseudomonas putida S12 for bioproduction of phenol from glucose.

Authors:  Nick J P Wierckx; Hendrik Ballerstedt; Jan A M de Bont; Jan Wery
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

4.  Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production.

Authors:  Nick J P Wierckx; Hendrik Ballerstedt; Jan A M de Bont; Johannes H de Winde; Harald J Ruijssenaars; Jan Wery
Journal:  J Bacteriol       Date:  2007-11-09       Impact factor: 3.490

5.  Biotransformation in double-phase systems: physiological responses of Pseudomonas putida DOT-T1E to a double phase made of aliphatic alcohols and biosynthesis of substituted catechols.

Authors:  Antonia Rojas; Estrella Duque; Andreas Schmid; Ana Hurtado; Juan-Luis Ramos; Ana Segura
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

6.  Bioproduction of p-hydroxystyrene from glucose by the solvent-tolerant bacterium Pseudomonas putida S12 in a two-phase water-decanol fermentation.

Authors:  Suzanne Verhoef; Nick Wierckx; R G Maaike Westerhof; Johannes H de Winde; Harald J Ruijssenaars
Journal:  Appl Environ Microbiol       Date:  2008-12-05       Impact factor: 4.792

7.  Genomotyping of Pseudomonas putida strains using P. putida KT2440-based high-density DNA microarrays: implications for transcriptomics studies.

Authors:  Hendrik Ballerstedt; Rita J M Volkers; Astrid E Mars; John E Hallsworth; Vitor A Martins dos Santos; Jaçek Puchalka; Joost van Duuren; Gerrit Eggink; Ken N Timmis; Jan A M de Bont; Jan Wery
Journal:  Appl Microbiol Biotechnol       Date:  2007-03-17       Impact factor: 4.813
  7 in total

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