Literature DB >> 1096818

Qualitative and quantitative assay of trichothecin: a mycotoxin produced by Trichothecium roseum.

W G Sorenson, M R Sneller, H W Larsh.   

Abstract

A method for quantitative determination of trichothecin in crude culture filtrates was presented. The method utilized an agar diffusion bioassay against Candida albicans, a colorimetric test involving a halochromatic reaction with sulfuric acid, and subsequent formation of blue color with methanol, and thin-layer chromatography of trichothecin and its dinitrophenylhydrazine derivative. A positive result in all three systems confirmed the presence of trichothecin. Quantitative results were generally in close agreement.

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Year:  1975        PMID： 1096818      PMCID： PMC187052          DOI： 10.1128/am.29.5.653-657.1975

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  8 in total

1.  [COLORIMETRIC DETERMINATION OF TRICHOTEZIN].

Authors:  V A POLTORAK
Journal:  Antibiotiki       Date:  1963-07

2.  A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.

Authors:  L J Wickerham
Journal:  J Bacteriol       Date:  1946-09       Impact factor: 3.490

3.  The isolation and chemical properties of trichothecin, an antifungal substance from Trichothecium roseum Link.

Authors:  G G Freeman; R I Morrison
Journal:  Biochem J       Date:  1949       Impact factor: 3.857

4.  Two classes of inhibitors of peptidyl transferase activity in eukaryotes.

Authors:  D Schindler
Journal:  Nature       Date:  1974-05-03       Impact factor: 49.962

5.  Biological and chemical detection of trichothecene mycotoxins of Fusarium species.

Authors:  Y Ueno; N Sato; K Ishii; K Sakai; H Tsunoda
Journal:  Appl Microbiol       Date:  1973-04

6.  Gas-liquid chromatography of mycotoxins of the trichothecene group.

Authors:  C O Ikediobi; I C Hsu; J R Bamburg; F M Strong
Journal:  Anal Biochem       Date:  1971-10       Impact factor: 3.365

7.  Toxigenicity of Trichothecium roseum Link: isolation and partial characterization of a toxic metabolite.

Authors:  J L Richard; G W Engstrom; A C Pier; L H Tiffany
Journal:  Mycopathol Mycol Appl       Date:  1969-12-29

8.  Mechanism of inhibition of eukaryotic protein synthesis by trichothecene fungal toxins.

Authors:  E Cundliffe; M Cannon; J Davies
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205
  8 in total
  3 in total

1.  Occurrence of trichothecin in wheat.

Authors:  K Ishii; J Kobayashi; Y Ueno; M Ichinoe
Journal:  Appl Environ Microbiol       Date:  1986-08       Impact factor: 4.792

2.  Screening of fungi for the presence of the trichodiene synthase encoding sequence by hybridization to the Tri5 gene cloned from Fusarium poae.

Authors:  C Fekete; A Logrieco; G Giczey; L Hornok
Journal:  Mycopathologia       Date:  1997       Impact factor: 2.574

3.  Comparative study of trichothecin, amphotericin B, and 5-fluorocytosine against Cryptococcus neoformans in vitro and in vivo.

Authors:  M R Sneller; A Hariri; W G Sorenson; H W Larsh
Journal:  Antimicrob Agents Chemother       Date:  1977-09       Impact factor: 5.191
  3 in total

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