BACKGROUND: Although the replacement of dietary saturated fat with unsaturated fat has been advocated to reduce the risk of cardiovascular disease, diets high in polyunsaturated fatty acids (PUFAs) could increase lipid peroxidation, potentially contributing to the pathology of atherosclerosis. OBJECTIVE: The objective of this study was to examine indexes of in vivo lipid peroxidation, including free F(2)-isoprostanes, malondialdehyde (MDA), and thiobarbituric acid reacting substances (TBARS), in the plasma of postmenopausal women taking dietary oil supplements rich in oleate, linoleate, and both eicosapentaenoic acid and docosahexaenoic acid. DESIGN:Fifteen postmenopausal women took 15 g sunflower oil/d, providing 12.3 goleate/d; safflower oil, providing 10.5 g linoleate/d; and fish oil, providing 2.0 g EPA/d and 1.4 g DHA/d in a 3-treatment crossover trial. RESULTS:Plasma free F(2)-isoprostane concentrations were lower after fish-oil supplementation than after sunflower-oil supplementation (P: = 0.003). When plasma free F(2)-isoprostane concentrations were normalized to plasma arachidonic acid concentrations, significant differences among the supplements were eliminated. Plasma MDA concentrations were lower after fish-oil supplementation than after sunflower-oil supplementation (P: = 0.04), whereas plasma TBARS were higher after fish-oil supplementation than after sunflower oil (P: = 0.003) and safflower oil (P: = 0.001) supplementation. When plasma MDA concentrations were normalized to plasma PUFA concentrations, significant differences were eliminated, but TBARS remained higher after fish-oil supplementation than after sunflower oil (P: = 0.01) and safflower-oil (P: = 0.0003) supplementation. CONCLUSIONS: With fish-oil supplementation, there was no evidence of increased lipid peroxidation when assessed by plasma F(2)-isoprostanes and MDA, although plasma TBARS was higher than with sunflower-oil and safflower-oil supplementation.
RCT Entities:
BACKGROUND: Although the replacement of dietary saturated fat with unsaturated fat has been advocated to reduce the risk of cardiovascular disease, diets high in polyunsaturated fatty acids (PUFAs) could increase lipid peroxidation, potentially contributing to the pathology of atherosclerosis. OBJECTIVE: The objective of this study was to examine indexes of in vivo lipid peroxidation, including free F(2)-isoprostanes, malondialdehyde (MDA), and thiobarbituric acid reacting substances (TBARS), in the plasma of postmenopausal women taking dietary oil supplements rich in oleate, linoleate, and both eicosapentaenoic acid and docosahexaenoic acid. DESIGN: Fifteen postmenopausal women took 15 g sunfloweroil/d, providing 12.3 g oleate/d; saffloweroil, providing 10.5 g linoleate/d; and fish oil, providing 2.0 g EPA/d and 1.4 g DHA/d in a 3-treatment crossover trial. RESULTS: Plasma free F(2)-isoprostane concentrations were lower after fish-oil supplementation than after sunflower-oil supplementation (P: = 0.003). When plasma free F(2)-isoprostane concentrations were normalized to plasma arachidonic acid concentrations, significant differences among the supplements were eliminated. Plasma MDA concentrations were lower after fish-oil supplementation than after sunflower-oil supplementation (P: = 0.04), whereas plasma TBARS were higher after fish-oil supplementation than after sunfloweroil (P: = 0.003) and saffloweroil (P: = 0.001) supplementation. When plasma MDA concentrations were normalized to plasma PUFA concentrations, significant differences were eliminated, but TBARS remained higher after fish-oil supplementation than after sunfloweroil (P: = 0.01) and safflower-oil (P: = 0.0003) supplementation. CONCLUSIONS: With fish-oil supplementation, there was no evidence of increased lipid peroxidation when assessed by plasma F(2)-isoprostanes and MDA, although plasma TBARS was higher than with sunflower-oil and safflower-oil supplementation.
Authors: Leon Darghosian; Marcia Free; Jie Li; Tebeb Gebretsadik; Aihua Bian; Ayumi Shintani; Brian F McBride; Joseph Solus; Ginger Milne; George H Crossley; David Thompson; Humberto Vidaillet; Henry Okafor; Dawood Darbar; Katherine T Murray; C Michael Stein Journal: Am J Cardiol Date: 2014-10-29 Impact factor: 2.778
Authors: Charles L Stebbins; James P Stice; C Michael Hart; Fiona N Mbai; Anne A Knowlton Journal: J Cardiovasc Pharmacol Ther Date: 2008-08-05 Impact factor: 2.457
Authors: A Y Thorlaksdottir; G V Skuladottir; A L Petursdottir; L Tryggvadottir; H M Ogmundsdottir; J E Eyfjord; J J Jonsson; I Hardardottir Journal: Lipids Date: 2006-02 Impact factor: 1.880
Authors: Patrick Wortman; Yuko Miyazaki; Nishan S Kalupahana; Suyeon Kim; Melissa Hansen-Petrik; Arnold M Saxton; Kate J Claycombe; Brynn H Voy; Jay Whelan; Naima Moustaid-Moussa Journal: Nutr Metab (Lond) Date: 2009-01-21 Impact factor: 4.169