Literature DB >> 10966447

Construction of a multihybrid display system: flagellar filaments carrying two foreign adhesive peptides.

J Tanskanen1, T K Korhonen, B Westerlund-Wikström.   

Abstract

A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented DeltafliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.

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Year:  2000        PMID： 10966447      PMCID： PMC92277          DOI： 10.1128/AEM.66.9.4152-4156.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  26 in total

Review 1.  Secretion and assembly of regular surface structures in Gram-negative bacteria.

Authors:  L A Fernández; J Berenguer
Journal:  FEMS Microbiol Rev       Date:  2000-01       Impact factor: 16.408

Review 2.  Peptide display on bacterial flagella: principles and applications.

Authors:  B Westerlund-Wikström
Journal:  Int J Med Microbiol       Date:  2000-07       Impact factor: 3.473

Review 3.  Phage-display technology--finding a needle in a vast molecular haystack.

Authors:  D J Rodi; L Makowski
Journal:  Curr Opin Biotechnol       Date:  1999-02       Impact factor: 9.740

4.  Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein.

Authors:  J M Betton; J P Jacob; M Hofnung; J K Broome-Smith
Journal:  Nat Biotechnol       Date:  1997-11       Impact factor: 54.908

5.  Flagellin domain that affects H antigenicity of Escherichia coli K-12.

Authors:  G Kuwajima
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

6.  Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria.

Authors:  R Fellay; J Frey; H Krisch
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  Functional expression of adhesive peptides as fusions to Escherichia coli flagellin.

Authors:  B Westerlund-Wikström; J Tanskanen; R Virkola; J Hacker; M Lindberg; M Skurnik; T K Korhonen
Journal:  Protein Eng       Date:  1997-11

8.  Cloning and expression of the gene for a fibronectin-binding protein from Staphylococcus aureus.

Authors:  J I Flock; G Fröman; K Jönsson; B Guss; C Signäs; B Nilsson; G Raucci; M Höök; T Wadström; M Lindberg
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598

9.  Intranuclear delivery of an antiviral peptide mediated by the B subunit of Escherichia coli heat-labile enterotoxin.

Authors:  A Loregian; E Papini; B Satin; H S Marsden; T R Hirst; G Palù
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-27       Impact factor: 11.205

10.  Display of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions.

Authors:  M Bendahmane; M Koo; E Karrer; R N Beachy
Journal:  J Mol Biol       Date:  1999-07-02       Impact factor: 5.469
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  2 in total

1.  Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum.

Authors:  Katariina Majander; Timo K Korhonen; Benita Westerlund-Wikström
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

2.  Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella.

Authors:  Ulrich Eckhard; Hina Bandukwala; Michael J Mansfield; Giada Marino; Jiujun Cheng; Iain Wallace; Todd Holyoak; Trevor C Charles; John Austin; Christopher M Overall; Andrew C Doxey
Journal:  Nat Commun       Date:  2017-09-12       Impact factor: 14.919
  2 in total

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