BACKGROUND: We report a method for detection of deep venous thrombosis with a technetium 99m-labeled peptide (DMP 444). The N-methyl-arginine-glycine-aspartic acid sequence on DMP 444 binds the glycoprotein IIb/IIIa receptor on activated platelets (inhibition constant [IC50] for fibrinogen binding = 6 nmol/L). METHODS: DMP 444 (23 to 27 mCi) was injected into 11 patients with clinical suspicion of deep venous thrombosis, diagnostic confirmation by ultrasound, and a positive D-dimer test result. Planar images in the anterior and posterior projections were obtained at 10 to 40 minutes, 50 to 80 minutes, and 120 to 150 minutes after injection. RESULTS: No clinically significant adverse effects were noted after DMP 444 administration. One patient (excluded from the analysis) withdrew consent, so image acquisition was not complete. By 10 to 40 minutes after injection, 8 of 10 patients demonstrated an area of increased activity that was clearly related to the abnormality noted on ultrasound. Most patients were taking warfarin (Coumadin) and heparin (n = 8) or heparin (n = 1) and warfarin (n = 1) alone at the time of the imaging. The average time from onset of symptoms to injection of DMP 444 was 5 days (range 1 to 18 days). CONCLUSION: These preliminary human studies indicate that DMP 444 is safe and may be of value in the diagnosis of deep venous thrombosis.
BACKGROUND: We report a method for detection of deep venous thrombosis with a technetium 99m-labeled peptide (DMP 444). The N-methyl-arginine-glycine-aspartic acid sequence on DMP 444 binds the glycoprotein IIb/IIIa receptor on activated platelets (inhibition constant [IC50] for fibrinogen binding = 6 nmol/L). METHODS:DMP 444 (23 to 27 mCi) was injected into 11 patients with clinical suspicion of deep venous thrombosis, diagnostic confirmation by ultrasound, and a positive D-dimer test result. Planar images in the anterior and posterior projections were obtained at 10 to 40 minutes, 50 to 80 minutes, and 120 to 150 minutes after injection. RESULTS: No clinically significant adverse effects were noted after DMP 444 administration. One patient (excluded from the analysis) withdrew consent, so image acquisition was not complete. By 10 to 40 minutes after injection, 8 of 10 patients demonstrated an area of increased activity that was clearly related to the abnormality noted on ultrasound. Most patients were taking warfarin (Coumadin) and heparin (n = 8) or heparin (n = 1) and warfarin (n = 1) alone at the time of the imaging. The average time from onset of symptoms to injection of DMP 444 was 5 days (range 1 to 18 days). CONCLUSION: These preliminary human studies indicate that DMP 444 is safe and may be of value in the diagnosis of deep venous thrombosis.
Authors: D C Farlow; M D Ezekowitz; S R Rao; C Martinez; D F Denny; S S Morse; J S Decho; F Wackers; E Strauss Journal: Am J Cardiol Date: 1989-08-01 Impact factor: 2.778
Authors: B J Rowbotham; P Carroll; A N Whitaker; I H Bunce; R G Cobcroft; M J Elms; P P Masci; P G Bundesen; D B Rylatt; A J Webber Journal: Thromb Haemost Date: 1987-02-03 Impact factor: 5.249
Authors: M A Bettmann; E W Salzman; D Rosenthal; P Clagett; G Davies; R Nebesar; K Rabinov; J Ploetz; J Skillman Journal: AJR Am J Roentgenol Date: 1980-06 Impact factor: 3.959
Authors: Timo Heidt; Friederike Deininger; Karlheinz Peter; Jürgen Goldschmidt; Annette Pethe; Christoph E Hagemeyer; Irene Neudorfer; Andreas Zirlik; Wolfgang A Weber; Christoph Bode; Philipp T Meyer; Martin Behe; Constantin von Zur Mühlen Journal: PLoS One Date: 2011-03-30 Impact factor: 3.240