Literature DB >> 1095486

Simplified assay for concanavalin A-dependent bacterial agglutination by using cell surface mutants.

H B Maruyama, M Arisawa, M Ono-Onitsuka.   

Abstract

Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 1095486      PMCID： PMC415218          DOI： 10.1128/iai.11.6.1320-1324.1975

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  7 in total

1.  Agglutination of bacterial spheroplast. 3. Relationship of labelled concanavalin A binding to the agglutinability.

Authors:  H B Maruyama
Journal:  J Biochem       Date:  1974-01       Impact factor: 3.387

Review 2.  The biochemistry of plant lectins (phytohemagglutinins).

Authors:  H Lis; N Sharon
Journal:  Annu Rev Biochem       Date:  1973       Impact factor: 23.643

3.  Biochemical and topographical studies on the cell surface of Escherichia coli. I. Isolation and characterization of detergent-sensitive mutants.

Authors:  M Ono-Onitsuka; H B Maruyama
Journal:  J Biochem       Date:  1974-09       Impact factor: 3.387

4.  A difference in the architecture of the surface membrane of normal and virally transformed cells.

Authors:  M M Burger
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

5.  Agglutination of bacterial spheroplasts: agglutination-dependent degradation of Escherichia coli ribosomal ribonucleic acid.

Authors:  H B Maruyama
Journal:  J Bacteriol       Date:  1973-07       Impact factor: 3.490

6.  Cell-surface changes of phosphate-deficient Escherichia coli as characterized by increased stability and decreased concanavalin A-agglutinability of the spheroplast.

Authors:  M Arisawa; H Maruyama
Journal:  J Biochem       Date:  1974-07       Impact factor: 3.387

7.  Agglutination of bacterial spheroplasts. I. Effect of concanavalin A.

Authors:  H B Maruyama
Journal:  Biochim Biophys Acta       Date:  1972-08-09
  7 in total
  2 in total

1.  Biochemical and topographical studies on Escherichia coli cell surface. IV. Giant spheroplast formation from a filamentous cell.

Authors:  M O Onitsuka; Y Rikihisa; H B Maruyama
Journal:  J Bacteriol       Date:  1979-05       Impact factor: 3.490

2.  Pleiotropic consequences of mutations towards antibiotic-hypersensitivity in Serratia marcescens.

Authors:  U Winkler; K B Heller; B Folle
Journal:  Arch Microbiol       Date:  1978-03       Impact factor: 2.552
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.