Literature DB >> 10954613

An extrachromosomal tetracycline-regulatable system for mammalian cells.

C R Sclimenti1, E J Baba, M P Calos.   

Abstract

We have modified the tetracycline-regulatable system so that all components are present on a stable extrachromosomal vector that can replicate in a wide variety of mammalian cells. An EBV/human ori vector is used to carry the system, overcoming the species specificity of conventional Epstein-Barr virus vectors. By placing the transcriptional transactivator gene under autoregulation, better induction characteristics are obtained. This system offers greater speed and sensitivity than previously reported methods. It can be applied within 3-4 weeks and produces an induction range of several hundred-fold with a low background.

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Year:  2000        PMID： 10954613      PMCID： PMC110717          DOI： 10.1093/nar/28.17.e80

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  An episomal vector for stable tetracycline-regulated gene expression.

Authors:  M Jost; C Kari; U Rodeck
Journal:  Nucleic Acids Res       Date:  1997-08-01       Impact factor: 16.971

2.  Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.

Authors:  M Gossen; H Bujard
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

3.  Replication control of autonomously replicating human sequences.

Authors:  S B Haase; M P Calos
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971

4.  Autonomous DNA replication in human cells is affected by the size and the source of the DNA.

Authors:  S S Heinzel; P J Krysan; C T Tran; M P Calos
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

5.  An autonomously replicating eukaryotic expression vector with a tetracycline-responsive promoter.

Authors:  Z Lang; J M Feingold
Journal:  Gene       Date:  1996-02-12       Impact factor: 3.688

6.  Isolation of human sequences that replicate autonomously in human cells.

Authors:  P J Krysan; S B Haase; M P Calos
Journal:  Mol Cell Biol       Date:  1989-03       Impact factor: 4.272

7.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

8.  Long-term gene expression from autonomously replicating vectors in mammalian cells.

Authors:  J G Wohlgemuth; S H Kang; G H Bulboaca; K A Nawotka; M P Calos
Journal:  Gene Ther       Date:  1996-06       Impact factor: 5.250

9.  Rapid retroviral delivery of tetracycline-inducible genes in a single autoregulatory cassette.

Authors:  A Hofmann; G P Nolan; H M Blau
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

10.  Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice.

Authors:  A Kistner; M Gossen; F Zimmermann; J Jerecic; C Ullmer; H Lübbert; H Bujard
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-01       Impact factor: 11.205
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  2 in total

Review 1.  rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines.

Authors:  Chinwe U Chukwudi
Journal:  Antimicrob Agents Chemother       Date:  2016-07-22       Impact factor: 5.191

2.  Down-regulation of osteopontin attenuates breast tumour progression in vivo.

Authors:  Goutam Chakraborty; Shalini Jain; Tushar V Patil; Gopal C Kundu
Journal:  J Cell Mol Med       Date:  2008-02-04       Impact factor: 5.310
  2 in total

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