| Literature DB >> 10952659 |
T Yanagita1, R Yamamoto, T Sugano, H Kobayashi, Y Uezono, H Yokoo, S Shiraishi, S I Minami, A Wada.
Abstract
In isolated rat uterine strips, adrenomedullin (AM) inhibited the spontaneous periodic contraction in a concentration-dependent manner (IC(50)=22.3+/-0.7 nM). The inhibitory effect of AM was prevented by either AM(22-52), a putative antagonist for AM receptors, or calcitonin gene-related peptide (CGRP)(8-37), a putative antagonist for CGRP receptors. AM also attenuated bradykinin (BK)-induced periodic uterine contraction, which was blocked by AM(22-52) or CGRP(8-37), whereas AM had no effect on the periodic contraction caused by oxytocin or prostaglandin F(2alpha) (PGF(2alpha)). RT-PCR analysis showed that mRNAs for calcitonin receptor-like receptor (CRLR), receptor-activity-modifying protein (RAMP)1, RAMP2 and RAMP3 were expressed in the rat uterus. These results demonstrate that AM selectively inhibits spontaneous and BK-induced periodic contraction via activating receptors for AM and CGRP.Entities:
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Year: 2000 PMID： 10952659 PMCID： PMC1572263 DOI： 10.1038/sj.bjp.0703500
Source DB: PubMed Journal: Br J Pharmacol ISSN： 0007-1188 Impact factor: 8.739