F Ropiquet1, D Giri, B Kwabi-Addo, K Schmidt, M Ittmann. 1. Department of Pathology, Baylor College of Medicine and Houston Department of Veterans Affairs Medical Center, TX 77030, USA.
Abstract
BACKGROUND: Fibroblast growth factors (FGFs) are known to play an important role in the growth of normal prostatic epithelial cells. FGF-10 is a secreted growth factor that binds to FGF receptor-2 IIIb, which is expressed in prostatic epithelial cells and thus can potentially act as a growth factor for these cells. Prior work has indicated that FGF10 may play an important role in the development of the rat prostate, but its role in the adult human prostate is unclear. METHODS: Expression of FGF10 in human prostate tissue and primary cultures of prostatic epithelial and stromal cells was assessed by reverse-transcriptase PCR (RT-PCR) and Northern blotting. Growth response to FGF10 was assessed by the addition of recombinant FGF-10 to primary cultures of prostatic epithelial and stromal cells. RESULTS: FGF10 is expressed at levels detectable by RT-PCR and can act as a growth factor for prostatic epithelial cells, but is not active as a growth factor for stromal cells. However, FGF10 is expressed at extremely low levels relative to FGF7, which has a similar biological activity. CONCLUSIONS: While FGF10 may play a role in prostatic development, it is unlikely to play a major role in prostate growth in normal or hyperplastic adult human prostate, due to its extremely low expression compared to FGF7. Copyright 2000 Wiley-Liss, Inc.
BACKGROUND: Fibroblast growth factors (FGFs) are known to play an important role in the growth of normal prostatic epithelial cells. FGF-10 is a secreted growth factor that binds to FGF receptor-2 IIIb, which is expressed in prostatic epithelial cells and thus can potentially act as a growth factor for these cells. Prior work has indicated that FGF10 may play an important role in the development of the rat prostate, but its role in the adult human prostate is unclear. METHODS: Expression of FGF10 in human prostate tissue and primary cultures of prostatic epithelial and stromal cells was assessed by reverse-transcriptase PCR (RT-PCR) and Northern blotting. Growth response to FGF10 was assessed by the addition of recombinant FGF-10 to primary cultures of prostatic epithelial and stromal cells. RESULTS:FGF10 is expressed at levels detectable by RT-PCR and can act as a growth factor for prostatic epithelial cells, but is not active as a growth factor for stromal cells. However, FGF10 is expressed at extremely low levels relative to FGF7, which has a similar biological activity. CONCLUSIONS: While FGF10 may play a role in prostatic development, it is unlikely to play a major role in prostate growth in normal or hyperplastic adult human prostate, due to its extremely low expression compared to FGF7. Copyright 2000 Wiley-Liss, Inc.
Authors: Zsofia Kote-Jarai; Ali Amin Al Olama; Graham G Giles; Gianluca Severi; Johanna Schleutker; Maren Weischer; Daniele Campa; Elio Riboli; Tim Key; Henrik Gronberg; David J Hunter; Peter Kraft; Michael J Thun; Sue Ingles; Stephen Chanock; Demetrius Albanes; Richard B Hayes; David E Neal; Freddie C Hamdy; Jenny L Donovan; Paul Pharoah; Fredrick Schumacher; Brian E Henderson; Janet L Stanford; Elaine A Ostrander; Karina Dalsgaard Sorensen; Thilo Dörk; Gerald Andriole; Joanne L Dickinson; Cezary Cybulski; Jan Lubinski; Amanda Spurdle; Judith A Clements; Suzanne Chambers; Joanne Aitken; R A Frank Gardiner; Stephen N Thibodeau; Dan Schaid; Esther M John; Christiane Maier; Walther Vogel; Kathleen A Cooney; Jong Y Park; Lisa Cannon-Albright; Hermann Brenner; Tomonori Habuchi; Hong-Wei Zhang; Yong-Jie Lu; Radka Kaneva; Ken Muir; Sara Benlloch; Daniel A Leongamornlert; Edward J Saunders; Malgorzata Tymrakiewicz; Nadiya Mahmud; Michelle Guy; Lynne T O'Brien; Rosemary A Wilkinson; Amanda L Hall; Emma J Sawyer; Tokhir Dadaev; Jonathan Morrison; David P Dearnaley; Alan Horwich; Robert A Huddart; Vincent S Khoo; Christopher C Parker; Nicholas Van As; Christopher J Woodhouse; Alan Thompson; Tim Christmas; Chris Ogden; Colin S Cooper; Aritaya Lophatonanon; Melissa C Southey; John L Hopper; Dallas R English; Tiina Wahlfors; Teuvo L J Tammela; Peter Klarskov; Børge G Nordestgaard; M Andreas Røder; Anne Tybjærg-Hansen; Stig E Bojesen; Ruth Travis; Federico Canzian; Rudolf Kaaks; Fredrik Wiklund; Markus Aly; Sara Lindstrom; W Ryan Diver; Susan Gapstur; Mariana C Stern; Roman Corral; Jarmo Virtamo; Angela Cox; Christopher A Haiman; Loic Le Marchand; Liesel Fitzgerald; Suzanne Kolb; Erika M Kwon; Danielle M Karyadi; Torben Falck Orntoft; Michael Borre; Andreas Meyer; Jürgen Serth; Meredith Yeager; Sonja I Berndt; James R Marthick; Briony Patterson; Dominika Wokolorczyk; Jyotsna Batra; Felicity Lose; Shannon K McDonnell; Amit D Joshi; Ahva Shahabi; Antje E Rinckleb; Ana Ray; Thomas A Sellers; Hui-Yi Lin; Robert A Stephenson; James Farnham; Heiko Muller; Dietrich Rothenbacher; Norihiko Tsuchiya; Shintaro Narita; Guang-Wen Cao; Chavdar Slavov; Vanio Mitev; Douglas F Easton; Rosalind A Eeles Journal: Nat Genet Date: 2011-07-10 Impact factor: 38.330