Attributes and dynamics of the endoplasmic reticulum in mammalian eggs.

The endoplasmic reticulum is a multifunctional continuous network of membrane-enclosed sacs and tubules that extends throughout the cell. The endoplasmic reticulum is the site of protein synthesis and assembly, as well as lipid and membrane synthesis. Additionally, the endoplasmic reticulum contains calcium pumps, intraluminal calcium storage proteins, and specific calcium-releasing channels. Thus, this membrane system plays a central role in intracellular signaling through the storage and release of calcium. At fertilization, the sperm triggers a large and dramatic release of calcium from the endoplasmic reticulum, which activates the egg to begin development. The ability of the egg to fully elevate calcium depends on biochemical and structural changes during oocyte maturation. The sensitivity of the calcium-releasing system increases and the endoplasmic reticulum is reorganized during maturation of the oocyte; together, these dynamic changes place a substantial calcium storage compartment just beneath the membrane, near the site of sperm-egg fusion. Localization of the calcium store may also contribute to the long-lasting calcium oscillations that are characteristic of mammalian fertilization. Examination of the endoplasmic reticulum in living eggs is leading to a better understanding of calcium release at fertilization.