Literature DB >> 10947985

Pig cloning by microinjection of fetal fibroblast nuclei.

A Onishi1, M Iwamoto, T Akita, S Mikawa, K Takeda, T Awata, H Hanada, A C Perry.   

Abstract

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.

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Year:  2000        PMID： 10947985     DOI： 10.1126/science.289.5482.1188

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  112 in total

1.  Assessment of the developmental totipotency of neural cells in the cerebral cortex of mouse embryo by nuclear transfer.

Authors:  Y Yamazaki; H Makino; K Hamaguchi-Hamada; S Hamada; H Sugino; E Kawase; T Miyata; M Ogawa; R Yanagimachi; T Yagi
Journal:  Proc Natl Acad Sci U S A       Date:  2001-11-06       Impact factor: 11.205

Review 2.  From teratocarcinomas to embryonic stem cells.

Authors:  Peter W Andrews
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2002-04-29       Impact factor: 6.237

Review 3.  Transgenic pigs as models for translational biomedical research.

Authors:  Bernhard Aigner; Simone Renner; Barbara Kessler; Nikolai Klymiuk; Mayuko Kurome; Annegret Wünsch; Eckhard Wolf
Journal:  J Mol Med (Berl)       Date:  2010-03-26       Impact factor: 4.599

4.  Establishment of a pig fibroblast-derived cell line for locus-directed transgene expression in cell cultures and blastocysts.

Authors:  Jannik E Jakobsen; Juan Li; Brian Moldt; Peter M Kragh; Henrik Callesen; Jens Michael Hertz; Lars Bolund; Arne Lund Jørgensen; Jacob Giehm Mikkelsen; Anders Lade Nielsen
Journal:  Mol Biol Rep       Date:  2010-03-25       Impact factor: 2.316

5.  Beneficial effect of young oocytes for rabbit somatic cell nuclear transfer.

Authors:  Fuliang Du; Jie Xu; Jifeng Zhang; Shaorong Gao; Mark G Carter; Chingli He; Li-Ying Sung; Sanjeev Chaubal; Rafael A Fissore; X Cindy Tian; Xiangzhong Yang; Y Eugene Chen
Journal:  Cloning Stem Cells       Date:  2009-03

Review 6.  Somatic Cell Nuclear Transfer Reprogramming: Mechanisms and Applications.

Authors:  Shogo Matoba; Yi Zhang
Journal:  Cell Stem Cell       Date:  2018-07-19       Impact factor: 24.633

7.  PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.

Authors:  Hirohide Uenishi; Tomoko Eguchi; Kohei Suzuki; Tetsuya Sawazaki; Daisuke Toki; Hiroki Shinkai; Naohiko Okumura; Noriyuki Hamasima; Takashi Awata
Journal:  Nucleic Acids Res       Date:  2004-01-01       Impact factor: 16.971

8.  Expression of X-linked genes in deceased neonates and surviving cloned female piglets.

Authors:  Le Jiang; Liangxue Lai; Melissa Samuel; Randall S Prather; Xiangzhong Yang; X Cindy Tian
Journal:  Mol Reprod Dev       Date:  2008-02       Impact factor: 2.609

9.  Effects of donor fibroblast cell type and transferred cloned embryo number on the efficiency of pig cloning.

Authors:  Zicong Li; Junsong Shi; Dewu Liu; Rong Zhou; Haiyu Zeng; Xiu Zhou; Ranbiao Mai; Shaofen Zeng; Lvhua Luo; Wanxian Yu; Shouquan Zhang; Zhenfang Wu
Journal:  Cell Reprogram       Date:  2012-12-20       Impact factor: 1.987

10.  Production of cloned NIBS (Nippon Institute for Biological Science) and α-1, 3-galactosyltransferase knockout MGH miniature pigs by somatic cell nuclear transfer using the NIBS breed as surrogates.

Authors:  Yoshiki Shimatsu; Kazuhiko Yamada; Wataru Horii; Atsushi Hirakata; Yuji Sakamoto; Shiori Waki; Junichi Sano; Toshiki Saitoh; Hisashi Sahara; Akira Shimizu; Hajime Yazawa; David H Sachs; Tetsuo Nunoya
Journal:  Xenotransplantation       Date:  2013-04-14       Impact factor: 3.907
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