Evaluation of the isoleucine requirement of the commercial layer in a corn-soybean meal diet.

An experiment was conducted with Hy-Line W36 hens to evaluate their Ile requirement in a corn-soybean meal diet. Five experimental diets were fed with Ile levels of 0.61, 0.58, 0.55, 0.52, and 0.49%. Supplemental amino acids (AA) were added to ensure that Ile was the first-limiting AA. Two diets with 0.55 and 0.52% Ile were also fed with higher levels of supplemental AA. A positive control (0.67% Ile) with only Met supplementation was fed, which had previously been shown to support maximum performance. All levels of Ile addition significantly increased egg production (EP), egg weight (EW), and egg contents (EC). Broken-line regression indicated a daily Ile requirement of 589.2, 601.2, and 601.4 mg per day for EP, EW, and EC, respectively, which indicated a requirement of 12.6 mg Ile per gram of EC.