Literature DB >> 10945459

A microfluidic cartridge to prepare spores for PCR analysis.

P Belgrader1, M Okuzumi, F Pourahmadi, D A Borkholder, M A Northrup.   

Abstract

A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.

Mesh:

Year:  2000        PMID: 10945459     DOI: 10.1016/s0956-5663(99)00060-3

Source DB:  PubMed          Journal:  Biosens Bioelectron        ISSN: 0956-5663            Impact factor:   10.618


  8 in total

Review 1.  Current and developing technologies for monitoring agents of bioterrorism and biowarfare.

Authors:  Daniel V Lim; Joyce M Simpson; Elizabeth A Kearns; Marianne F Kramer
Journal:  Clin Microbiol Rev       Date:  2005-10       Impact factor: 26.132

2.  Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs.

Authors:  Vicki A Luna; Debra King; Carisa Davis; Tony Rycerz; Matthew Ewert; Andrew Cannons; Philip Amuso; Jacqueline Cattani
Journal:  J Clin Microbiol       Date:  2003-03       Impact factor: 5.948

3.  Multiscale Multiphysics and Multidomain Models I: Basic Theory.

Authors:  Guo-Wei Wei
Journal:  J Theor Comput Chem       Date:  2013-12       Impact factor: 0.939

4.  Variational multiscale models for charge transport.

Authors:  Guo-Wei Wei; Qiong Zheng; Zhan Chen; Kelin Xia
Journal:  SIAM Rev Soc Ind Appl Math       Date:  2012-11-08       Impact factor: 10.780

5.  DNA-based bioanalytical microsystems for handheld device applications.

Authors:  Thomas Ming-Hung Lee; I-Ming Hsing
Journal:  Anal Chim Acta       Date:  2005-07-07       Impact factor: 6.558

Review 6.  On-Chip Detection of the Biomarkers for Neurodegenerative Diseases: Technologies and Prospects.

Authors:  Chao Song; Suya Que; Lucas Heimer; Long Que
Journal:  Micromachines (Basel)       Date:  2020-06-28       Impact factor: 2.891

7.  Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores.

Authors:  Dimitra N Stratis-Cullum; Guy D Griffin; Joel Mobley; Tuan Vo-Dinh
Journal:  Anal Bioanal Chem       Date:  2008-01-27       Impact factor: 4.142

8.  Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC).

Authors:  Pascal Basselet; Grzegorz Wegrzyn; Sven-Olof Enfors; Magdalena Gabig-Ciminska
Journal:  Microb Cell Fact       Date:  2008-10-13       Impact factor: 5.328

  8 in total

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