Literature DB >> 10933390

Monomeric and oligomeric complexes of the B cell antigen receptor.

W W Schamel1, M Reth.   

Abstract

The current structural model of the B cell antigen receptor (BCR) describes it as a symmetric protein complex in which one membrane-bound immunoglobulin molecule (mIg) is noncovalently bound on each side by an Ig-alpha/Ig-beta heterodimer. Using peptide-tagged Ig-alpha proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE), and biosynthetical labeling of B cells, we find that the mIg:Ig-alpha/Ig-beta complex has a stoichiometry of 1:1 and not 1:2. An anti-Flag stimulation of B cells coexpressing Flag-tagged and wild-type Ig-alpha proteins results in the phosphorylation of both Ig-alpha proteins, suggesting that on the surface of living B cells, several BCR monomers are in contact with each other. A BN-PAGE analysis after limited detergent lysis provides further evidence for an oligomeric BCR structure.

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Year:  2000        PMID： 10933390     DOI： 10.1016/s1074-7613(00)00003-0

Source DB:  PubMed          Journal:  Immunity        ISSN： 1074-7613            Impact factor:   31.745


  67 in total

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Journal:  J Immunol       Date:  2002-05-01       Impact factor: 5.422

2.  Heavy-chain only antibodies derived from dromedary are secreted and displayed by mouse B cells.

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Journal:  Immunology       Date:  2003-05       Impact factor: 7.397

Review 3.  Models of signal transduction through the B-cell antigen receptor.

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Journal:  Immunology       Date:  2003-12       Impact factor: 7.397

Review 4.  B-cell receptor: from resting state to activate.

Authors:  Bebhinn Treanor
Journal:  Immunology       Date:  2012-05       Impact factor: 7.397

5.  The growth of B cell receptor microcluster is a universal response of B cells encountering antigens with different motion features.

Authors:  Zhengpeng Wan; Wanli Liu
Journal:  Protein Cell       Date:  2012-07-10       Impact factor: 14.870

6.  Structural and functional studies of Igalphabeta and its assembly with the B cell antigen receptor.

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Journal:  Structure       Date:  2010-08-11       Impact factor: 5.006

Review 7.  The cytoskeleton coordinates the early events of B-cell activation.

Authors:  Naomi E Harwood; Facundo D Batista
Journal:  Cold Spring Harb Perspect Biol       Date:  2011-02-01       Impact factor: 10.005

8.  Nanoscale organization and dynamics of the siglec CD22 cooperate with the cytoskeleton in restraining BCR signalling.

Authors:  Francesca Gasparrini; Christoph Feest; Andreas Bruckbauer; Pieta K Mattila; Jennifer Müller; Lars Nitschke; Dennis Bray; Facundo D Batista
Journal:  EMBO J       Date:  2015-12-15       Impact factor: 11.598

9.  The transmembrane tyrosine of micro-heavy chain is required for BCR destabilization and entry of antigen into clathrin-coated vesicles.

Authors:  Jin Hyang Kim; Jennifer A Rutan; Barbara J Vilen
Journal:  Int Immunol       Date:  2007-11-01       Impact factor: 4.823

10.  Change in the Molecular Dimension of a RAGE-Ligand Complex Triggers RAGE Signaling.

Authors:  Jing Xue; Michaele Manigrasso; Matteo Scalabrin; Vivek Rai; Sergey Reverdatto; David S Burz; Daniele Fabris; Ann Marie Schmidt; Alexander Shekhtman
Journal:  Structure       Date:  2016-08-11       Impact factor: 5.006
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