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Literature DB >> 10930583

Production of polyomavirus structural protein VP1 in yeast cells and its interaction with cell structures.

Z Palková¹, T Adamec, D Liebl, J Stokrová, J Forstová.

Abstract

The gene for mouse polyomavirus major structural protein VP1 was expressed in Saccharomyces cerevisiae from the inducible GAL7 promoter. VP1 pseudocapsids were purified from cell lysates. Their subpopulation contained fragments of host DNA, which, in contrast to those of VP1 pseudocapsids produced in insect cells, did not assemble with cellular histones into pseudonucleocores. VP1 pseudocapsids accumulated in the yeast cell nuclei. A strong interaction of VP1 with tubulin fibres of the mitotic spindle was observed. The fibres of spindles were larger in diameter, apparently due to tight VP1 binding. Substantial growth inhibition of yeast cells producing VP1 was observed.

Entities: Gene Species

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Year: 2000 PMID： 10930583 DOI： 10.1016/s0014-5793(00)01787-7

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

7 in total

1. Caveolae are involved in the trafficking of mouse polyomavirus virions and artificial VP1 pseudocapsids toward cell nuclei.

Authors: Z Richterová; D Liebl; M Horák; Z Palková; J Stokrová; P Hozák; J Korb; J Forstová
Journal: J Virol Date: 2001-11 Impact factor: 5.103

2. Disulfide linkage and structure of highly stable yeast-derived virus-like particles of murine polyomavirus.

Authors: Claudia Simon; Thomas Klose; Sabine Herbst; Bong Gyoon Han; Andrea Sinz; Robert M Glaeser; Milton T Stubbs; Hauke Lilie
Journal: J Biol Chem Date: 2014-02-24 Impact factor: 5.157

3. Design strategies to address the effect of hydrophobic epitope on stability and in vitro assembly of modular virus-like particle.

Authors: Alemu Tekewe; Natalie K Connors; Anton P J Middelberg; Linda H L Lua
Journal: Protein Sci Date: 2016-06-13 Impact factor: 6.725

4. Minor capsid proteins of simian virus 40 are dispensable for nucleocapsid assembly and cell entry but are required for nuclear entry of the viral genome.

Authors: Akira Nakanishi; Noriko Itoh; Peggy P Li; Hiroshi Handa; Robert C Liddington; Harumi Kasamatsu
Journal: J Virol Date: 2007-01-31 Impact factor: 5.103

Production of polyomavirus structural protein VP1 in yeast cells and its interaction with cell structures.

1. Caveolae are involved in the trafficking of mouse polyomavirus virions and artificial VP1 pseudocapsids toward cell nuclei.

2. Disulfide linkage and structure of highly stable yeast-derived virus-like particles of murine polyomavirus.

3. Design strategies to address the effect of hydrophobic epitope on stability and in vitro assembly of modular virus-like particle.

4. Minor capsid proteins of simian virus 40 are dispensable for nucleocapsid assembly and cell entry but are required for nuclear entry of the viral genome.

Review 5. Contribution of yeast models to virus research.

6. Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast.

Review 7. Microtubules in Polyomavirus Infection.