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Literature DB >> 10929812

A protein biosensor for lactate.

S D'Auria¹, Z Gryczynski, I Gryczynski, M Rossi, J R Lakowicz.

Abstract

Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein.

Entities: Chemical

Mesh：

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Year: 2000 PMID： 10929812 PMCID： PMC6986380 DOI： 10.1006/abio.2000.4611

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

25 in total

1. Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria, XI. Engineering thermostability and activity of lactate dehydrogenases from bacilli.

Authors: F Zülli; R Schneiter; R Urfer; H Zuber
Journal: Biol Chem Hoppe Seyler Date: 1991-05

2. Low-frequency modulation sensors using nanosecond fluorophores.

Authors: J R Lakowicz; F N Castellano; J D Dattelbaum; L Tolosa; G Rao; I Gryczynski
Journal: Anal Chem Date: 1998-12-15 Impact factor: 6.986

3. Intrinsic molecular fluorescence of lactate dehydrogenase: an analytical alternative for enzymic determination of pyruvate.

Authors: S de Marcos; J Galbán; C Alonsa; J R Castillo
Journal: Analyst Date: 1997-04 Impact factor: 4.616

4. A microdialysis fibre based sampler for flow injection analysis: determination of L-lactate in biofluids by an electrochemically synthesised bilayer membrane based biosensor.

Authors: F Palmisano; D Centonze; M Quinto; P G Zambonin
Journal: Biosens Bioelectron Date: 1996 Impact factor: 10.618

2. Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.

Authors: Lisa Goers; Catherine Ainsworth; Cher Hui Goey; Cleo Kontoravdi; Paul S Freemont; Karen M Polizzi
Journal: Biotechnol Bioeng Date: 2017-02-23 Impact factor: 4.530

2 in total

A protein biosensor for lactate.

1. Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria, XI. Engineering thermostability and activity of lactate dehydrogenases from bacilli.

2. Low-frequency modulation sensors using nanosecond fluorophores.

3. Intrinsic molecular fluorescence of lactate dehydrogenase: an analytical alternative for enzymic determination of pyruvate.

4. A microdialysis fibre based sampler for flow injection analysis: determination of L-lactate in biofluids by an electrochemically synthesised bilayer membrane based biosensor.

5. Association between blood lactate and acid-base status and mortality in ventilated babies.

6. Assessment of the lactate biosensor methodology.

7. Lactate compared with pH analysis at fetal scalp blood sampling: a prospective randomised study.

8. [Detection of ligand-induced conformation changes in lactate dehydrogenase by using fluorescent probes].

9. The use of auramine O to study ligand binding and subunit cooperativity of lactate dehydrogenase.

10. Glucose sensor for low-cost lifetime-based sensing using a genetically engineered protein.

1. Fluorescence characterization of the hydrophobic pocket of cyclophilin B.

2. Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.