Suppression of adrenocorticotropic hormone release by stimulation of the nerve fibres in the anterior pituitary.

With the recent revelation of considerable peptidergic innervation of the anterior pituitary in several mammalian species, including man, it becomes imperative to elucidate the physiological significance of such a morphological entity. We addressed this issue by employing an anterior pituitary slice in vitro superfusion system coupled with electrical field stimulation. Anterior pituitary slices of 0.8 mm were perfused with Krebs-Ringer bicarbonate-bovine serum albumin buffer in a superfusion chamber for 30 min before electrical field stimulation. A square current of 30 mA, 10 Hz and 0.5 ms was then applied for 10 min. The perfusate was collected every 10 min and measured for adrenocorticotropic hormone (ACTH) by radio-immunoassay. It was found that under the experimental condition the basal release of ACTH was suppressed by electrical field stimulation of the nerve fibres in the anterior pituitary. Furthermore, vasopressin was added as a secretagogue. The suppression of ACTH by electrical field stimulation became even more marked. This is the first physiological evidence of the effect of stimulation of the nerve fibres innervating the anterior pituitary on its secretory activity.