PURPOSE: To prospectively characterize serum antibody responses in high-risk corneal allografts. METHODS: Orthotopic and heterotopic C3H (H-2k) corneas were transplanted to CB6F1 (H-2b/d) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement. RESULTS: In high-risk donor-host combinations, 100% corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes. CONCLUSIONS: Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.
PURPOSE: To prospectively characterize serum antibody responses in high-risk corneal allografts. METHODS: Orthotopic and heterotopic C3H (H-2k) corneas were transplanted to CB6F1 (H-2b/d) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement. RESULTS: In high-risk donor-host combinations, 100% corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes. CONCLUSIONS: Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.