A simple single-step immunoimmobilisation method for the detection of Salmonella in the presence of large numbers of other bacteria.

A single-step disk immunoimmobilisation method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more senstitive than a standard method for detection of Salmonella under the experimental conditons used. The time required for detection is shortened to 24-48 h.