Literature DB >> 10927875

Detection of Theileria annulata carrier cattle by PCR.

K C Roy1, D Ray, G C Bansal, R K Singh.   

Abstract

A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR, in which 8 cattle revealed presence of T. annulata in stained blood smear under microscope.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 10927875

Source DB:  PubMed          Journal:  Indian J Exp Biol        ISSN： 0019-5189            Impact factor:   0.818


  6 in total

1.  Establishment and application of a qPCR diagnostic method for Theileria annulata.

Authors:  Tianxing Cao; Junlong Liu; Zhi Li; Kangyan Shi; Miao Shi; Youquan Li; Guiquan Guan; Hong Yin; Jianxun Luo
Journal:  Parasitol Res       Date:  2022-01-26       Impact factor: 2.289

2.  Survey on infection rate, vectors and molecular identification of Theileria annulata in cattle from North West, Iran.

Authors:  Jafar Arjmand Yamchi; Mousa Tavassoli
Journal:  J Parasit Dis       Date:  2014-12-20

3.  Detection of theileriosis in cattle and buffaloes by polymerase chain reaction.

Authors:  V R Kundave; A K Patel; P V Patel; J J Hasnani; C G Joshi
Journal:  J Parasit Dis       Date:  2013-11-19

4.  Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle.

Authors:  Gaurav Charaya; N K Rakha; Sushila Maan; Aman Kumar; Tarun Kumar; Ricky Jhambh
Journal:  Vet World       Date:  2016-09-30

5.  Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle.

Authors:  H C Chauhan; B K Patel; A G Bhagat; M V Patel; S I Patel; S H Raval; H H Panchasara; M D Shrimali; A C Patel; B S Chandel
Journal:  Vet World       Date:  2015-11-28

6.  Prevalence of carrier state theileriosis in lactating cows.

Authors:  Niranjana Sahoo; Bikash Kumar Behera; Hemant Kumar Khuntia; Manojita Dash
Journal:  Vet World       Date:  2017-12-14
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.