Literature DB >> 10922481

Enzyme kinetics of hevamine, a chitinase from the rubber tree Hevea brasiliensis.

E Bokma1, T Barends, A C Terwissch van Scheltingab, B W Dijkstr, J J Beintema.   

Abstract

The enzyme kinetics of hevamine, a chitinase from the rubber tree Hevea brasiliensis, were studied in detail with a new enzyme assay. In this assay, the enzyme reaction products were derivatized by reductive coupling to a chromophore. Products were separated by HPLC and the amount of product was calculated by peak integration. Penta-N-acetylglucosamine (penta-nag) and hexa-N-acetylglucosamine (hexa-nag) were used as substrates. Hexa-nag was more efficiently converted than penta-nag, which is an indication that hevamine has at least six sugar binding sites in the active site. Tetra-N-acetylglucosamine (tetra-nag) and allosamidin were tested as inhibitors. Allosamidin was found to be a competitive inhibitor with a K(i) of 3.1 microM. Under the conditions tested, tetra-nag did not inhibit hevamine.

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Year:  2000        PMID： 10922481     DOI： 10.1016/s0014-5793(00)01833-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  6 in total

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Authors:  D M van Aalten; D Komander; B Synstad; S Gåseidnes; M G Peter; V G Eijsink
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-31       Impact factor: 11.205

2.  High-resolution structures of a chitinase complexed with natural product cyclopentapeptide inhibitors: mimicry of carbohydrate substrate.

Authors:  Douglas R Houston; Kazuro Shiomi; Noriko Arai; Satoshi Omura; Martin G Peter; Andreas Turberg; Bjørnar Synstad; Vincent G H Eijsink; Daan M F van Aalten
Journal:  Proc Natl Acad Sci U S A       Date:  2002-07-01       Impact factor: 11.205

3.  Effect of different carbon sources on endochitinase production by Colletotrichum gloeosporioides.

Authors:  R F Souza; R M A Soares; R P Nascimento; R R R Coelho; R C Gomes
Journal:  Curr Microbiol       Date:  2005-06-16       Impact factor: 2.188

4.  A class III chitinase without disulfide bonds from the fern, Pteris ryukyuensis: crystal structure and ligand-binding studies.

Authors:  Yoshihito Kitaoku; Naoyuki Umemoto; Takayuki Ohnuma; Tomoyuki Numata; Toki Taira; Shohei Sakuda; Tamo Fukamizo
Journal:  Planta       Date:  2015-05-22       Impact factor: 4.116

Review 5.  Function, distribution, and annotation of characterized cellulases, xylanases, and chitinases from CAZy.

Authors:  Stanley T C Nguyen; Hannah L Freund; Joshua Kasanjian; Renaud Berlemont
Journal:  Appl Microbiol Biotechnol       Date:  2018-01-22       Impact factor: 4.813

6.  Acetazolamide-based fungal chitinase inhibitors.

Authors:  Alexander W Schüttelkopf; Ludovic Gros; David E Blair; Julie A Frearson; Daan M F van Aalten; Ian H Gilbert
Journal:  Bioorg Med Chem       Date:  2010-10-08       Impact factor: 3.641
  6 in total

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