Literature DB >> 1092150

Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.

D A Keefer, W E Stumpf, P Petrusz, M Sar.   

Abstract

A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized "gonadotropes". Most, but not all, "gonadotropes" show significant nuclear concentration of radioactivity. A large percentage of non-"gonadotropes" also show nuclear uptake of label.

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Year:  1975        PMID： 1092150     DOI： 10.1002/aja.1001420110

Source DB:  PubMed          Journal:  Am J Anat        ISSN： 0002-9106


  6 in total

1.  Quantitative autoradiographic assessment of 3H-estradiol uptake in immunocytochemically characterized pituitary cells.

Authors:  D A Keefer; W E Stumpf; P Petrusz
Journal:  Cell Tissue Res       Date:  1976-02-06       Impact factor: 5.249

2.  Evaluation of the ability of Tween 80, dimethylsulphoxide and Triton X-100 to enhance immunocytochemical staining of autoradiograms.

Authors:  D C Herbert; F J Weaker; P J Sheridan
Journal:  Histochem J       Date:  1982-01

3.  Dihydrotestosterone induces a sexual dimorphism in estrogen uptake by specific anterior pituitary cell types in vivo.

Authors:  D A Keefer; N Dordai; R Mallonga; K Ziegler; P Shughrue; P Ramirez
Journal:  Cell Tissue Res       Date:  1987-08       Impact factor: 5.249

4.  In vivo estrogen uptake by individual cell types of the rat anterior pituitary after short-term castration-adrenalectomy.

Authors:  D A Keefer
Journal:  Cell Tissue Res       Date:  1980       Impact factor: 5.249

5.  Simultaneous localization of steroid and peptide hormones in rat pituitary by combined thaw-mount autoradiography and immunohistochemistry: localization of dihydrotestosterone in gonadotropes, thyrotropes and pituicytes.

Authors:  M Sar; W E Stumpf
Journal:  Cell Tissue Res       Date:  1979-11       Impact factor: 5.249

6.  Does neonatal androgen modulate uptake and retention of 3H-estradiol in gonadotrophs and lactotrophs?

Authors:  H Nogami; F Yoshimura; D C Herbert; P J Sheridan
Journal:  Cell Tissue Res       Date:  1985       Impact factor: 5.249
  6 in total

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