L Zhang1, J Jiang, R Tan. 1. Department of Clinical Biochemistry, Chongqing University of Medical Science.
Abstract
OBJECTIVE: In order to search for new tumor differentiation inducer, the effects of matrine of Chinese herbal origin on differentiation of K562 cells and its mechanism were studied. METHODS: Telomerase activity was determined by PCR-ELISA in untreated or treated K562 cells, cell cycle phases were analyzed by fluorescence-activated cell sorter analysis. RESULTS: Telomerase activity of K562 cells was significantly inhibited when treated with matrine, and cell cycle of the treated K562 cells was changed with marked decrease in S phase. CONCLUSION: Matrine can induce differentiation of K562 cells, the mechanism of which might be related to the inhibition of telomerase activity and cell cycle arrest.
OBJECTIVE: In order to search for new tumor differentiation inducer, the effects of matrine of Chinese herbal origin on differentiation of K562 cells and its mechanism were studied. METHODS: Telomerase activity was determined by PCR-ELISA in untreated or treated K562 cells, cell cycle phases were analyzed by fluorescence-activated cell sorter analysis. RESULTS: Telomerase activity of K562 cells was significantly inhibited when treated with matrine, and cell cycle of the treated K562 cells was changed with marked decrease in S phase. CONCLUSION: Matrine can induce differentiation of K562 cells, the mechanism of which might be related to the inhibition of telomerase activity and cell cycle arrest.