H Huang1, X Pan, L Yu. 1. Department of Otolaryngology, Changhai Hospital, Second Military Medical University.
Abstract
OBJECTIVE: To investigate the effect of EB virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cell. METHODS: Expression vector for BHRF1 was constructed and transfected into nsaopharyngeal carcinoma cell line, CNE2. The alteration of biologic behaviour of the transfected cells was tested after 60Co radiation. RESULTS: BHRF1 expression could inhibit the expression of proliferative cell nuclear antigen, redistribute cell cycle, decrease cell sensitivity to radiation, and enhance their survival ability. CONCLUSION: It is suggested that BHRF1 expression can increase the apoptotic resistance induced by radiation.
OBJECTIVE: To investigate the effect of EB virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cell. METHODS: Expression vector for BHRF1 was constructed and transfected into nsaopharyngeal carcinoma cell line, CNE2. The alteration of biologic behaviour of the transfected cells was tested after 60Co radiation. RESULTS:BHRF1 expression could inhibit the expression of proliferative cell nuclear antigen, redistribute cell cycle, decrease cell sensitivity to radiation, and enhance their survival ability. CONCLUSION: It is suggested that BHRF1 expression can increase the apoptotic resistance induced by radiation.