J Liu1, Z Tian, R Sun. 1. Shandong Cancer Biotherapy Center, Shandong Academy of Medical Sciences, Jinan.
Abstract
OBJECTIVE: To observe the expression of Th1 versus Th2 type cytokines in human tumor cells. METHODS: The gene expression of Th1/Th2 cytokines in human tumor cells was detected by RT-PCR and RNA dot hybridization using IL-2 and IFN gamma as Th1 type cytokine genes, IL-4, IL-10 and IL-13 as Th2 type cytokine genes. RESULTS: There were predominant expressions of Th2 type cytokines in 20 different human tumor cell lines including 11 solid tumor and 9 hematogenous tumor cells. CONCLUSION: Predominant expression of Th2 type cytokines by tumor cells may be related to the evasion of tumor cells from immune surveillance.
OBJECTIVE: To observe the expression of Th1 versus Th2 type cytokines in humantumor cells. METHODS: The gene expression of Th1/Th2 cytokines in humantumor cells was detected by RT-PCR and RNA dot hybridization using IL-2 and IFN gamma as Th1 type cytokine genes, IL-4, IL-10 and IL-13 as Th2 type cytokine genes. RESULTS: There were predominant expressions of Th2 type cytokines in 20 different humantumor cell lines including 11 solid tumor and 9 hematogenous tumor cells. CONCLUSION: Predominant expression of Th2 type cytokines by tumor cells may be related to the evasion of tumor cells from immune surveillance.