Literature DB >> 1091638

Purification and properties of rabbit reticulocyte protein synthesis elongation factor 2.

W C Merrick, W M Kemper, J A Kantor, W F Anderson.   

Abstract

A homogeneous preparation of elongation factor 2 (EF-2) has been obtained from rabbit reticulocytes. EF-2, purified 1,960-fold, appears to be active as a single polypeptide chain with a molecular weight of approximately 100,000 based upon the following determinations: sodium dodecyl sulfate gel electrophoresis (95,000); sedimentation equilibrium centrifugation (112,000); gel filtration (97,000); ADP-ribosylation (103,000). The amino acid composition of rabbit reticulocyte EF-2 is almost identical with that of rat liver EF-2. The unknown amino acid in rat liver EF-2 which can be ADP-ribosylated appears also to be present in rabbit reticulocyte EF-2. A comparison of the amino acid composition of rabbit reticulocyte and rat liver EF-2 with Escherichia coli EF-G shows a high degree of similarity with only four amino acids differing by more than 10% (alanine, lysine, cysteine, and leucine).

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Year:  1975        PMID： 1091638

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  10 in total

1.  Purification and characterization of two initiation factors required for maximal activity of a highly fractionated globin mRNA translation system.

Authors:  B Safer; S L Adams; W M Kemper; K W Berry; M Lloyd; W C Merrick
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

2.  Pseudomonas aeruginosa exoenzyme S: an adenosine diphosphate ribosyltransferase distinct from toxin A.

Authors:  B H Iglewski; J Sadoff; M J Bjorn; E S Maxwell
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

3.  Isolation and distribution of elongation factor 2 in eggs and embryos of sea urchins.

Authors:  Z Yablonka-Reuveni; M B Hille
Journal:  Biochemistry       Date:  1983-10-25       Impact factor: 3.162

4.  Microsomal and cytosolic fractions of guinea pig hepatocytes contain 100-kilodalton GTP-binding proteins reactive with antisera against alpha subunits of stimulatory and inhibitory heterotrimeric GTP-binding proteins.

Authors:  D Udrisar; M Rodbell
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

5.  Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with GTP-binding proteins.

Authors:  K Kohno; T Uchida; H Ohkubo; S Nakanishi; T Nakanishi; T Fukui; E Ohtsuka; M Ikehara; Y Okada
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

6.  Identification of the gene encoding the mitochondrial elongation factor G in mammals.

Authors:  C Barker; A Makris; C Patriotis; S E Bear; P N Tsichlis
Journal:  Nucleic Acids Res       Date:  1993-06-11       Impact factor: 16.971

7.  Biochemical evidence of translational infidelity and decreased peptidyltransferase activity by a sarcin/ricin domain mutation of yeast 25S rRNA.

Authors:  Panagiotis Panopoulos; John Dresios; Dennis Synetos
Journal:  Nucleic Acids Res       Date:  2004-10-11       Impact factor: 16.971

8.  Purification of elongation factor 2 from human placenta and evidence of its fragmentation patterns in various eukaryotic sources.

Authors:  A Giovane; L Servillo; L Quagliuolo; C Balestrieri
Journal:  Biochem J       Date:  1987-06-01       Impact factor: 3.857

9.  Mechanism of action of Pseudomonas aeruginosa exotoxin Aiadenosine diphosphate-ribosylation of mammalian elongation factor 2 in vitro and in vivo.

Authors:  B H Iglewski; P V Liu; D Kabat
Journal:  Infect Immun       Date:  1977-01       Impact factor: 3.441

Review 10.  The Regulation of Translation in Alphavirus-Infected Cells.

Authors:  Luis Carrasco; Miguel Angel Sanz; Esther González-Almela
Journal:  Viruses       Date:  2018-02-08       Impact factor: 5.048
  10 in total

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