Literature DB >> 10910205

Determination of neurotoxin 3-N-oxalyl-2,3-diaminopropionic acid and non-protein amino acids in Lathyrus sativus by precolumn derivatization with 1-fluoro-2,4-dinitrobenzene.

F Wang1, X Chen, Q Chen, X Qin, Z Li.   

Abstract

A rapid and simple method is presented for determining neuro-excitatory nonprotein amino acid 3-N-oxalyl-2,3-diaminopropionic acid (beta-ODAP) and non-protein amino acids in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatography method (RP HPLC) for the separation of the derivatives in the pmol range is reported. The RP HPLC method and a colorimetric method were compared for measuring ODAP.

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Year:  2000        PMID： 10910205     DOI： 10.1016/s0021-9673(00)00264-8

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  3 in total

1.  Simple Detection Methods for Antinutritive Factor β-ODAP Present in Lathyrus sativus L. by High Pressure Liquid Chromatography and Thin Layer Chromatography.

Authors:  Bidisha Ghosh; Joy Mitra; Saikat Chakraborty; Jagannath Bhattacharyya; Anirban Chakraborty; Soumitra Kumar Sen; Muniasamy Neerathilingam
Journal:  PLoS One       Date:  2015-11-02       Impact factor: 3.240

2.  An Improved HILIC HPLC-MS/MS Method for the Determination of β-ODAP and Its α Isomer in Lathyrus sativus.

Authors:  Andreia Bento-Silva; Letice Gonçalves; Elsa Mecha; Filipe Pereira; Maria Carlota Vaz Patto; Maria do Rosário Bronze
Journal:  Molecules       Date:  2019-08-22       Impact factor: 4.411

3.  A novel potentiometric sensor for determination of neurotoxin β-N-oxalyl-L-α, β-diaminopropionic acid.

Authors:  Omer Isildak; Furkan Saymaz; Ahmet Karadag; Nesrin Okumus Korkmaz; Azade Attar
Journal:  Biomed Res Int       Date:  2014-05-20       Impact factor: 3.411
  3 in total

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