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Literature DB >> 10908345

Silanized nucleic acids: a general platform for DNA immobilization.

Abstract

We have developed a method for simultaneous deposition and covalent cross-linking of oligonucleotide or PCR products on unmodified glass surfaces. By covalently conjugating an active silyl moiety onto oligonucleotides or cDNA in solutions we have generated a new class of modified nucleic acids, namely silanized nucleic acids. Such silanized molecules can be immobilized instantly onto glass surfaces after manual or automated deposition. This method provides a simple and rapid, yet very efficient, solution to the immobilization of prefabricated oligonucleotides and DNA for chip production.

Entities: Chemical

Mesh：

Substances：

Year: 2000 PMID： 10908345 PMCID： PMC102671 DOI： 10.1093/nar/28.14.e71

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. DNA chip technology.

Authors: K M Kurian; C J Watson; A H Wyllie
Journal: J Pathol Date: 1999-02 Impact factor: 7.996

2. Electrospray deposition as a method for mass fabrication of mono- and multicomponent microarrays of biological and biologically active substances.

Authors: V N Morozov
Journal: Anal Chem Date: 1999-08-01 Impact factor: 6.986

3. Genome-wide analysis of DNA copy-number changes using cDNA microarrays.

Authors: J R Pollack; C M Perou; A A Alizadeh; M B Eisen; A Pergamenschikov; C F Williams; S S Jeffrey; D Botstein; P O Brown
Journal: Nat Genet Date: 1999-09 Impact factor: 38.330

4. Single nucleotide polymorphic discrimination by an electronic dot blot assay on semiconductor microchips.

Authors: P N Gilles; D J Wu; C B Foster; P J Dillon; S J Chanock
Journal: Nat Biotechnol Date: 1999-04 Impact factor: 54.908

Review 5. Making and reading microarrays.

Authors: V G Cheung; M Morley; F Aguilar; A Massimi; R Kucherlapati; G Childs
Journal: Nat Genet Date: 1999-01 Impact factor: 38.330

6. Aminooxy functionalized oligonucleotides: preparation, on-support derivatization, and postsynthetic attachment to polymer support.

Authors: H Salo; P Virta; H Hakala; T P Prakash; A M Kawasaki; M Manoharan; H Lönnberg
Journal: Bioconjug Chem Date: 1999 Sep-Oct Impact factor: 4.774

7. Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array.

Authors: S Singh-Gasson; R D Green; Y Yue; C Nelson; F Blattner; M R Sussman; F Cerrina
Journal: Nat Biotechnol Date: 1999-10 Impact factor: 54.908

8. Determination of ancestral alleles for human single-nucleotide polymorphisms using high-density oligonucleotide arrays.

Authors: J G Hacia; J B Fan; O Ryder; L Jin; K Edgemon; G Ghandour; R A Mayer; B Sun; L Hsie; C M Robbins; L C Brody; D Wang; E S Lander; R Lipshutz; S P Fodor; F S Collins
Journal: Nat Genet Date: 1999-06 Impact factor: 38.330

9. Detection of salmonella in poultry using a silicon chip-based biosensor.

Authors: K Dill; L H Stanker; C R Young
Journal: J Biochem Biophys Methods Date: 1999-08-30

Review 10. High density synthetic oligonucleotide arrays.

Authors: R J Lipshutz; S P Fodor; T R Gingeras; D J Lockhart
Journal: Nat Genet Date: 1999-01 Impact factor: 38.330

11 in total

1. Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries.

Authors: K Lindroos; U Liljedahl; M Raitio; A C Syvänen
Journal: Nucleic Acids Res Date: 2001-07-01 Impact factor: 16.971

2. Construction of oligonucleotide arrays on a glass surface using a heterobifunctional reagent, N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine (NTMTA).

Authors: P Kumar; Jyoti Choithani; K C Gupta
Journal: Nucleic Acids Res Date: 2004-06-02 Impact factor: 16.971

3. Molecular charge contact biosensing based on the interaction of biologically modified magnetic beads with an ion-sensitive field effect transistor.

Authors: Yuuya Miyazawa; Toshiya Sakata
Journal: Eur Biophys J Date: 2014-03-05 Impact factor: 1.733

Silanized nucleic acids: a general platform for DNA immobilization.

1. DNA chip technology.

2. Electrospray deposition as a method for mass fabrication of mono- and multicomponent microarrays of biological and biologically active substances.

3. Genome-wide analysis of DNA copy-number changes using cDNA microarrays.

4. Single nucleotide polymorphic discrimination by an electronic dot blot assay on semiconductor microchips.

Review 5. Making and reading microarrays.

6. Aminooxy functionalized oligonucleotides: preparation, on-support derivatization, and postsynthetic attachment to polymer support.

7. Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array.

8. Determination of ancestral alleles for human single-nucleotide polymorphisms using high-density oligonucleotide arrays.

9. Detection of salmonella in poultry using a silicon chip-based biosensor.

Review 10. High density synthetic oligonucleotide arrays.

1. Minisequencing on oligonucleotide microarrays: comparison of immobilisation chemistries.

2. Construction of oligonucleotide arrays on a glass surface using a heterobifunctional reagent, N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine (NTMTA).

3. Molecular charge contact biosensing based on the interaction of biologically modified magnetic beads with an ion-sensitive field effect transistor.

4. Device considerations for development of conductance-based biosensors.

5. Chemical nanoprinting: a novel method for fabricating DNA microchips.

6. Synergistic effects of epoxy- and amine-silanes on microarray DNA immobilization and hybridization.

Review 7. Immobilization techniques for microarray: challenges and applications.

8. DNA Microarray for Rapid Detection and Identification of Food and Water Borne Bacteria: From Dry to Wet Lab.

9. Use of functional gene arrays for elucidating in situ biodegradation.

10. Improving signal intensities for genes with low-expression on oligonucleotide microarrays.