| Literature DB >> 10905708 |
E Gimeno1, A I Castellote, R M Lamuela-Raventós, M C de la Torre, M C López-Sabater.
Abstract
A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).Entities:
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Year: 2000 PMID: 10905708 DOI: 10.1016/s0021-9673(00)00219-3
Source DB: PubMed Journal: J Chromatogr A ISSN: 0021-9673 Impact factor: 4.759