J Fomina1, F Darroudi, J J Boei, A T Natarajan. 1. MGC, Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Centre, The Netherlands. Fomina@Rullf2.MedFac.LeidenUniv.NL
Abstract
PURPOSE: To discriminate precisely between radiation-induced complete and incomplete chromosome exchanges using chromosome painting together with the detection of the centromeres and telomeres in one FISH assay. MATERIALS AND METHODS: Human lymphocytes were exposed in vitro to X-rays at a dose of 4 Gy. Chromosome aberrations were analysed using the FISH technique in combination with a whole chromosome-specific DNA probe for chromosome 8, human pan-centromeric DNA and telomeric PNA probes. RESULTS: The combined FISH assay has improved the resolution of detecting chromosomal exchanges in human lymphocytes. Results indicate that the frequency of observed incomplete exchange patterns was 21% when telomeric signals were ignored during the analysis. When the telomeric signals were included in the analysis a large proportion of apparently incomplete exchange patterns appeared complete and should be re-classified. The percentage of true incomplete exchanges was found to be less than 5%. CONCLUSION: The combination of chromosome painting and the detection of centromeres and telomeres enable unequivocal discrimination between incomplete and complete exchanges. The fraction of true incomplete exchanges observed in X-irradiated human lymphocytes was found to be low in comparison with previous reports in the literature.
PURPOSE: To discriminate precisely between radiation-induced complete and incomplete chromosome exchanges using chromosome painting together with the detection of the centromeres and telomeres in one FISH assay. MATERIALS AND METHODS:Human lymphocytes were exposed in vitro to X-rays at a dose of 4 Gy. Chromosome aberrations were analysed using the FISH technique in combination with a whole chromosome-specific DNA probe for chromosome 8, human pan-centromeric DNA and telomeric PNA probes. RESULTS: The combined FISH assay has improved the resolution of detecting chromosomal exchanges in human lymphocytes. Results indicate that the frequency of observed incomplete exchange patterns was 21% when telomeric signals were ignored during the analysis. When the telomeric signals were included in the analysis a large proportion of apparently incomplete exchange patterns appeared complete and should be re-classified. The percentage of true incomplete exchanges was found to be less than 5%. CONCLUSION: The combination of chromosome painting and the detection of centromeres and telomeres enable unequivocal discrimination between incomplete and complete exchanges. The fraction of true incomplete exchanges observed in X-irradiated human lymphocytes was found to be low in comparison with previous reports in the literature.
Authors: Elena V Gostjeva; Vera Koledova; Aoy Tomita-Mitchell; Michael Mitchell; Mary A Goetsch; Susannah Varmuza; Janna N Fomina; Firouz Darroudi; William G Thilly Journal: Organogenesis Date: 2009-10 Impact factor: 2.500
Authors: Alice J Sigurdson; Mina Ha; Michael Hauptmann; Parveen Bhatti; Radim J Sram; Olena Beskid; E Janet Tawn; Caroline A Whitehouse; Carita Lindholm; Mimako Nakano; Yoshiaki Kodama; Nori Nakamura; Irena Vorobtsova; Ursula Oestreicher; Günther Stephan; Lee C Yong; Manfred Bauchinger; Ernst Schmid; Hai Won Chung; Firouz Darroudi; Laurence Roy; Phillipe Voisin; Joan F Barquinero; Gordon Livingston; David Blakey; Isamu Hayata; Wei Zhang; Chunyan Wang; L Michelle Bennett; L Gayle Littlefield; Alan A Edwards; Ruth A Kleinerman; James D Tucker Journal: Mutat Res Date: 2008-02-02 Impact factor: 2.433