Nucleotide sequence and synthesis of infectious RNA from cloned cDNA of broad bean wilt virus 2 RNA 2*.

The complete nucleotide sequence of broad bean wilt virus -2 (BBWV-2) RNA 2 was determined. The sequence is 3593 nucleotides in length, and contains a single open reading frame which has the potential to encode a 119 kDa polyprotein. Direct amino acid sequencing of the virus coat proteins suggests that the polyprotein is processed to produce three proteins, 52 kDa, 44 kDa and 22 kDa in size. A comparison of several fabaviruses in terms of nucleotide sequence and amino acid sequences showed that BBWV-2 isolates display high sequence identity. A full-length cDNA constructed under the control of the T7 promoter could be successfully transcribed to produce biologically active RNA. This is the first report on generating infectious transcripts of BBWV-2 RNA 2.