Literature DB >> 10890014

Analysis of genetically modified plant gene expression using GUS fluorimetry.

K M Gartland1, A T McHugh, S Vitha, K Benes, R J Irvine, J S Gartland.   

Abstract

A fluorimetric assay method for the analysis of beta-glucuronidase (GUS) reporter gene expression in genetically modified plants is described. Optimization of this method for woody plants and a statistical approach suitable for comparisons of gene expression in different transformants or tissues of the same plant is described. Example data from elm (Ulmus procera) SR4 regenerant plants, shown to be genetically modified by PCR and DNA-DNA hybridizations, in which higher GUS expression levels are found in stems than in leaves demonstrates the utility of this approach.

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Year:  2000        PMID： 10890014     DOI： 10.1385/MB:14:3:235

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  8 in total

1.  A simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences.

Authors:  B Hanson; D Engler; Y Moy; B Newman; E Ralston; N Gutterson
Journal:  Plant J       Date:  1999-09       Impact factor: 6.417

Review 2.  The GUS reporter gene system.

Authors:  R A Jefferson
Journal:  Nature       Date:  1989-12-14       Impact factor: 49.962

3.  Intrinsic GUS-like activities in seed plants.

Authors:  C Y Hu; P P Chee; R H Chesney; J H Zhou; P D Miller; W T O'Brien
Journal:  Plant Cell Rep       Date:  1990-06       Impact factor: 4.570

4.  Mutations that suppress the thermosensitivity of green fluorescent protein.

Authors:  K R Siemering; R Golbik; R Sever; J Haseloff
Journal:  Curr Biol       Date:  1996-12-01       Impact factor: 10.834

5.  beta-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other gram-negative bacteria.

Authors:  K J Wilson; A Sessitsch; J C Corbo; K E Giller; A D Akkermans; R A Jefferson
Journal:  Microbiology       Date:  1995-07       Impact factor: 2.777

6.  GFP in plants.

Authors:  J Haseloff; B Amos
Journal:  Trends Genet       Date:  1995-08       Impact factor: 11.639

7.  Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.

Authors:  J Haseloff; K R Siemering; D C Prasher; S Hodge
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-18       Impact factor: 11.205

8.  Expression of a bacterial gene in plants mediated by infectious geminivirus DNA.

Authors:  A Ward; P Etessami; J Stanley
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598
  8 in total
  2 in total

1.  Molecular cloning, characterization, and function analysis of a mevalonate pyrophosphate decarboxylase gene from Ganoderma lucidum.

Authors:  Liang Shi; Lei Qin; Yingjie Xu; Ang Ren; Xing Fang; Dashuai Mu; Qi Tan; Mingwen Zhao
Journal:  Mol Biol Rep       Date:  2011-12-28       Impact factor: 2.316

2.  Somatic embryogenesis in elm.

Authors:  E Corredoira; A M Vieitez; A Ballester
Journal:  Ann Bot       Date:  2002-05       Impact factor: 4.357
  2 in total

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