B Fichtlscherer1, A Mülsch. 1. Institut für Kardiovaskuläre Physiologie, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, D-60590 Frankfurt, Germany.
Abstract
PURPOSE: To assess the influence of several nitrosyl-iron complexes on proton nuclear spin relaxation rates to establish a magnetic resonance (MR) imaging technique for nitric oxide. MATERIALS AND METHODS: The influence of aqueous phantom solutions of nitrosyl-iron complexes on proton relaxation rates was analyzed for signal enhancement at conventional 1.5-T MR imaging. To induce formation of nitrosyl-iron complexes in a biologic tissue, isolated rat liver was perfused with a saline solution of the NO donor sodium nitroprusside (SNP), and the MR signal intensity was examined thereafter. RESULTS: All investigated nitrosyl-iron complexes shortened the longitudinal, or T1, and transverse, or T2, relaxation times in a concentration-dependent fashion. Relaxivities were highest with a dinitrosyl-iron complex bound to albumin and with a water-soluble mononitrosyl-iron dithiocarbamate complex. The contrast properties of 240 micromol/L of a paramagnetic nitrosyl-iron complex were sufficient to substantially enhance the signal intensity of SNP-perfused rat livers at hydrogen 1 MR imaging. CONCLUSION: Nitrosyl-iron complexes exhibit a contrast effect at MR imaging that can be exploited for NO imaging in living animals and patients with conventional (1)H MR imaging techniques.
PURPOSE: To assess the influence of several nitrosyl-iron complexes on proton nuclear spin relaxation rates to establish a magnetic resonance (MR) imaging technique for nitric oxide. MATERIALS AND METHODS: The influence of aqueous phantom solutions of nitrosyl-iron complexes on proton relaxation rates was analyzed for signal enhancement at conventional 1.5-T MR imaging. To induce formation of nitrosyl-iron complexes in a biologic tissue, isolated rat liver was perfused with a saline solution of the NO donorsodium nitroprusside (SNP), and the MR signal intensity was examined thereafter. RESULTS: All investigated nitrosyl-iron complexes shortened the longitudinal, or T1, and transverse, or T2, relaxation times in a concentration-dependent fashion. Relaxivities were highest with a dinitrosyl-iron complex bound to albumin and with a water-soluble mononitrosyl-iron dithiocarbamate complex. The contrast properties of 240 micromol/L of a paramagnetic nitrosyl-iron complex were sufficient to substantially enhance the signal intensity of SNP-perfused rat livers at hydrogen 1 MR imaging. CONCLUSION:Nitrosyl-iron complexes exhibit a contrast effect at MR imaging that can be exploited for NO imaging in living animals and patients with conventional (1)H MR imaging techniques.
Authors: Ali Barandov; Souparno Ghosh; Nan Li; Benjamin B Bartelle; Jade I Daher; Michael L Pegis; Hannah Collins; Alan Jasanoff Journal: ACS Sens Date: 2020-06-02 Impact factor: 7.711