Literature DB >> 10886775

Intron-tagged epitope: a tool for facile detection and purification of proteins expressed in Agrobacterium-transformed plant cells.

A Ferrando1, R Farràs, J Jásik, J Schell, C Koncz.   

Abstract

Epitope tagging provides a useful tool for immunological detection and cellular localization of proteins in vivo. Using T-DNA-mediated transformation, the detection of epitope-tagged proteins in planta is currently feasible only in transgenic plants, because an artificial expression of cDNA and gene constructs driven by plant promoters in bacteria obscures an early detection of epitope-tagged proteins in Agrobacterium-infected plant cells. We have developed a method for labelling plant coding sequences with intron-tagged epitope-coding domains that are not processed in Agrobacterium. Here we show that the expression of HA-epitope-tagged constructs encoding beta-glucuronidase and S-phase kinase-associated (AtSKP1/ASK1) proteins can be specifically and exclusively detected in cultured Arabidopsis cells as early as five days after Agrobacterium infection. This epitope-tagging approach offers an unlimited source of transformed material for purification and localization of proteins expressed individually or simultaneously in Agrobacterium-transformed plant cells.

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Year:  2000        PMID： 10886775     DOI： 10.1046/j.1365-313x.2000.00763.x

Source DB:  PubMed          Journal:  Plant J        ISSN： 0960-7412            Impact factor:   6.417


  35 in total

1.  Use of epitope tags for routine analysis of transgene expression.

Authors:  C M Alarcon; A R Umthun; J C Register
Journal:  Transgenic Res       Date:  2001-06       Impact factor: 2.788

2.  Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labelling in plants cells.

Authors:  A Ferrando; Z Koncz-Kálmán; R Farràs; A Tiburcio; J Schell; C Koncz
Journal:  Nucleic Acids Res       Date:  2001-09-01       Impact factor: 16.971

3.  Arabidopsis E2Fc functions in cell division and is degraded by the ubiquitin-SCF(AtSKP2) pathway in response to light.

Authors:  Juan Carlos del Pozo; Maria Beatrice Boniotti; Crisanto Gutierrez
Journal:  Plant Cell       Date:  2002-12       Impact factor: 11.277

4.  Transcriptional control of aspartate kinase expression during darkness and sugar depletion in Arabidopsis: involvement of bZIP transcription factors.

Authors:  Shai Ufaz; Vijaya Shukla; Yulia Soloveichik; Yelena Golan; Frank Breuer; Zsuzsa Koncz; Gad Galili; Csaba Koncz; Aviah Zilberstein
Journal:  Planta       Date:  2011-01-30       Impact factor: 4.116

5.  The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants.

Authors:  László Bakó; Masaaki Umeda; Antonio F Tiburcio; Jeff Schell; Csaba Koncz
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-04       Impact factor: 11.205

6.  The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis.

Authors:  Lionel Navarro; Cyril Zipfel; Owen Rowland; Ingo Keller; Silke Robatzek; Thomas Boller; Jonathan D G Jones
Journal:  Plant Physiol       Date:  2004-06-04       Impact factor: 8.340

7.  Deregulated copper transport affects Arabidopsis development especially in the absence of environmental cycles.

Authors:  Nuria Andrés-Colás; Ana Perea-García; Sergi Puig; Lola Peñarrubia
Journal:  Plant Physiol       Date:  2010-03-24       Impact factor: 8.340

8.  AtTPK4, an Arabidopsis tandem-pore K+ channel, poised to control the pollen membrane voltage in a pH- and Ca2+-dependent manner.

Authors:  D Becker; D Geiger; M Dunkel; A Roller; A Bertl; A Latz; A Carpaneto; P Dietrich; M R G Roelfsema; C Voelker; D Schmidt; B Mueller-Roeber; K Czempinski; R Hedrich
Journal:  Proc Natl Acad Sci U S A       Date:  2004-10-25       Impact factor: 11.205

9.  The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis.

Authors:  Akie Shimotohno; Chikage Umeda-Hara; Katerina Bisova; Hirofumi Uchimiya; Masaaki Umeda
Journal:  Plant Cell       Date:  2004-10-14       Impact factor: 11.277

10.  The plastid protein THYLAKOID FORMATION1 and the plasma membrane G-protein GPA1 interact in a novel sugar-signaling mechanism in Arabidopsis.

Authors:  Jirong Huang; J Philip Taylor; Jin-Gui Chen; Joachim F Uhrig; Danny J Schnell; Tsuyoshi Nakagawa; Kenneth L Korth; Alan M Jones
Journal:  Plant Cell       Date:  2006-03-31       Impact factor: 11.277
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